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  5. CD63 Antibody (YA530)

CD63 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 26 kDa, targeting to CD63. It can be used for WB,IHC-P assays with tag free, in the background of Human, Mouse.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

Publications Citing Use of MCE CD63 Antibody (YA530)

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CD63 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 26 kDa, targeting to CD63. It can be used for WB,IHC-P assays with tag free, in the background of Human, Mouse.
Background

CD63: Involved in pigment cell differentiation and regulation of vascular endothelial growth factor signaling pathway. Located in late endosome and lysosome. Is expressed in blood vessel; brain; eye anterior segment; and intestine. Orthologous to human CD63 (CD63 molecule). [provided by Alliance of Genome Resources, Apr 2022]
Tag

Free
Gene ID
SwissProt ID
Synonyms
CD63 antigen; CD63
Molecular Weight

Predicted band size: 26 kDa; Observed band size: 40-70 kDa
Purity

affinity purified
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Cardiovascular
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse
Dilution Ratio

WB: 1:500-1:1,000 ;IHC: 1:50-1:100

  • Western blot analysis of extracts from RAW264.7(lane 2(20μg) and RAW264.7(lane 3(40μg) using CD63 Antibody (HY-P80604) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Application

WB, IHC-P
Appearance

Liquid
Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

Synthetic peptide corresponding to Mouse CD63.The exact sequence is proprietary to MCE.
Database

Entrez Gene: 967 Human ; 12512 Mouse ;

SwissProt: P08962 Human ; P41731 Mouse ;

OMIM: 155740 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

CD63 Antibody (YA530) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
CD63 Antibody (YA530)
Cat. No.:
HY-P80604
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