CD31 Antibody (YA5642)

CD31 Antibody (YA5642) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD31. It can be applicated for IHC-P, WB, ICC/IF, ELISA assays, in the background of human.

CD31 is a cell adhesion molecule essential for leukocyte transendothelial migration (TEM) under inflammatory conditions. Its Tyr-690 residue critically regulates TEM by facilitating PECAM1 trafficking to/from the lateral border recycling compartment (LBRC) and directing LBRC membrane targeting around migrating leukocytes. CD31 mediates endothelial cell-cell adhesion via homophilic interactions and promotes neutrophil TEM through heterophilic binding with CD177. Homophilic ligation transmits a detachment signal to prevent phagocytosis of viable leukocytes by macrophages, while tethering apoptotic leukocytes to phagocytes (the detachment signal is disabled in apoptosis). It modulates BDKRB2 activation and regulates bradykinin/hyperosmotic shock-induced ERK1/2 signaling in endothelial cells (By similarity: may confer atherosclerosis susceptibility but lacks anti-apoptotic protection).

P16284

Predicted band size: 83 kDa; Observed band size: 130 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

IHC: 1:200-1000; WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

IHC-P, WB, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Synthesized peptide derived from human CD31 AA range: 100-200