CD31 Antibody (YA806)

CD31 Antibody (YA806) is a non-conjugated and Mouse origined monoclonal antibody about 83 kDa, targeting to CD31 (2D3). It can be used for WB, IF-Cell, IF-Tissue, IHC-P, mIHC assays with tag free, in the background of Human, Mouse.

CD31 (2D3): The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Free

P16284

Predicted band size: 83 kDa;Observed band size: 130 kDa

affinity purified

Cell junction. Cell membrane. Membrane.

Non-conjugated

Unmodified

AB_3102929

Cardiovascular

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:1,000-1:5,000；000 ;IF-Cell: 1:50-1:200；IF-Tissue: 1:50-1:200；IHC-P: 1:500-1:2,000；mIHC: 1:1,000-1:3,000

• 
  Western blot analysis of extracts from THP-1 (lane 2(20μg) and THP-1 (lane 3(40μg)using CD31 (HY-P80599) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80599, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling CD31 with CD31 antibody (HY-P80599) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD31 antibody (HY-P80599) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IF-Tissue, mIHC

Liquid

Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Endogenous

Synthetic peptide (KLH-coupled) within C-terminal residues of human CD31.