CD147 Antibody

CD147 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD147.

CD147: Predicted to enable cell-cell adhesion mediator activity; signaling receptor activity; and virus receptor activity. Involved in neural retina development; photoreceptor cell maintenance; and spermatogenesis. Located in several cellular components, including acrosomal membrane; photoreceptor inner segment; and photoreceptor outer segment. Is expressed in several structures, including alimentary system; brain; early conceptus; reproductive system; and sensory organ. Orthologous to human BSG (basigin (Ok blood group)). [provided by Alliance of Genome Resources, Apr 2022]

Free

P18572

Predicted band size: 42 kDa;Observed band size: 38-58 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102621

Immunology

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Mouse

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IP: 1:20 ;FC: 1:50-1:100

• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, FC, IP

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Mouse CD147.The exact sequence is proprietary to MCE.