Caspase-3 Antibody (YA557)

Caspase-3 Antibody (YA557) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-3.

CASP3 is a thiol protease that functions as a major effector caspase in the execution phase of apoptosis. Activated by initiator caspases (such as CASP8, CASP9, or CASP10) through cleavage, it mediates apoptosis by catalyzing the cleavage of numerous proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at the '216-Asp-|-Gly-217' bond. By similarity, it cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Additionally, CASP3 cleaves and activates caspase-6 (CASP6), caspase-7 (CASP7), and caspase-9 (CASP9), while also cleaving and inactivating interleukin-18 (IL18). It is involved in the cleavage of huntingtin and triggers cell adhesion in sympathetic neurons through RET cleavage. Under oxidative stress, CASP3 cleaves and inhibits serine/threonine-protein kinase AKT1. During virus-induced apoptosis, it acts as an inhibitor of type I interferon production by cleaving antiviral proteins CGAS, IRF3, and MAVS, preventing cytokine overproduction. CASP3 also participates in pyroptosis by cleaving and activating gasdermin-E (GSDME). Furthermore, it cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8, and XKR9, promoting phosphatidylserine exposure on apoptotic cell surfaces. Finally, following inhibition of BIRC6-caspase binding by DIABLO/SMAC, CASP3 cleaves BIRC6.

Free

P42574

Predicted band size: 32 kDa; Observed band size: 17 kDa

Protein A affinity purified.

Cytoplasm

Non-conjugated

Unmodified

AB_3102061

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500; ICC/IF: 1:50; IHC-P: 1:50; FC:1:20-1:50; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela (lane 2(20μg), Jurkat (lane 3(20μg) and HEK293 (lane 4(20μg) using Caspase-3(HY-P80046) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Caspase-3 with Caspase-3 Antibody (HY-P80046)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Caspase-3 Antibody (HY-P80046) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Caspase-3.AA range:60-100.