P-Cadherin Antibody (YA1535)

Cat. No.: HY-P81790: Data Sheet
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P-Cadherin Antibody (YA1535) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to P-Cadherin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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P-Cadherin Antibody (YA1535) Featured Recommendations:

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

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Description	P-Cadherin Antibody (YA1535) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to P-Cadherin.
Background	The related gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to related generate the mature glycoprotein. The calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The related gene is located in a related gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of The protein is observed in cervical adenocarcinomas. Mutations in The related gene are associated with hypotrichosis with juvenile macular dystrophy and ectodermal dysplasia, ectrodactyly, and macular dystrophy syndrome (EEMS).
Gene ID	1001 [NCBI]
SwissProt ID	P22223
Synonyms	CDHP; HJMD; PCAD; CDH3 ; Cadherin-3; Placental cadherin (P-cadherin)
Molecular Weight	Predicted band size: 97 kDa; Observed band size: 130-150 kDa
Purity	Affinity Purified
Conjugation	Non-conjugated
Modification	Unmodified
RRID	AB_3103893
Research Field	Signal Transduction
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality	Recombinant, Monoclonal
Host	Rabbit
Reactivity	Human, Mouse, Rat
Dilution Ratio	WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20 Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using P-Cadherin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature. Immunocytochemistry analysis of Hela cells labeling P-Cadherin with P-Cadherin Antibody (HY-P81790) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with P-Cadherin Antibody (HY-P81790) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Immunocytochemistry analysis of NIH3T3 cells labeling P-Cadherin with P-Cadherin Antibody (HY-P81790) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with P-Cadherin Antibody (HY-P81790)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using P-Cadherin Antibody (YA1535). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81790, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using P-Cadherin Antibody (YA1535). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81790, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application	WB, IHC-F, IHC-P, ICC/IF, IP
Appearance	Liquid
Formulation	Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	A synthetic peptide of human pan Cadherin
Database	Entrez Gene: 1001 Human ; 1002 Human ; 1003 Human ; 1004 Human ; 1005 Human ; 1006 Human ; 1008 Human ; 1009 Human ; 1012 Human ; 1014 Human ; 1015 Human ; 999 Human ; SwissProt: P12830 Human ; P19022 Human ; P22223 Human ; P33151 Human ; P55283 Human ; P39038 Mouse ; OMIM: 137215 Human
Documentation	Data Sheet (234 KB) SDS (252 KB) User Guide for Antibodies (1077 KB)

P-Cadherin Antibody (YA1535) Related Classifications

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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P-Cadherin Antibody (YA1535)

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