BLNK Antibody (YA4749)

BLNK Antibody (YA4749) is a mouse-derived and non-conjugated monoclonal antibody, targeting to BLNK. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse.

BLNK, a central linker protein, operates downstream of the B-cell receptor (BCR), serving as a crucial bridge that connects the SYK kinase to various signaling pathways and regulates diverse biological outcomes in B-cell function and development. Its functional spectrum includes playing a role in the activation of ERK/EPHB2, MAP kinase p38, and JNK, as well as modulating AP1 activation. BLNK is pivotal for the activation of NF-kappa-B and NFAT, contributing significantly to BCR-mediated PLCG1 and PLCG2 activation, Ca(2+) mobilization, and the trafficking of the BCR to late endosomes. Notably, BLNK is dispensable for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. Additionally, it may be crucial for the RAC1-JNK pathway and plays a critical role in orchestrating the pro-B cell to pre-B cell transition. BLNK's involvement extends to potential participation in BCR-induced B-cell apoptosis, forming essential associations with PLCG1, VAV1, NCK1, VAV3, PLCG2, GRB2, CD79A, and phosphorylated and activated SYK, thereby intricately connecting with key elements in B-cell signaling pathways.

Q8WV28

Predicted band size: 50 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:500-1:2000; IHC: 1:200-1:1000; ICC/IF: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human BLNK expressed in E. Coli.