beta Catenin Antibody (YA938)

beta Catenin Antibody (YA938) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to beta Catenin.

Beta-catenin, a pivotal player in the canonical Wnt signaling pathway, dynamically regulates cellular processes in response to Wnt ligands. In the absence of Wnt, beta-catenin participates in a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK3B, promoting its phosphorylation and ubiquitination, leading to subsequent degradation by the proteasome. Upon Wnt activation, beta-catenin accumulates in the nucleus, acting as a coactivator for transcription factors of the TCF/LEF family, thereby activating Wnt-responsive genes. This multifaceted protein also plays a role in cell adhesion, acting as a component of an E-cadherin:catenin adhesion complex. Beyond these functions, beta-catenin emerges as a negative regulator of centrosome cohesion and exhibits roles in insulin internalization, inhibition of anoikis, and promotion of neurogenesis. It modulates chondrocyte differentiation, positively regulates odontoblast differentiation during tooth germ formation, and interacts with various proteins, including regulators and effectors of Wnt signaling, highlighting its central role in diverse cellular processes. Beta-catenin's complex network of interactions underscores its significance in orchestrating cellular responses to intricate signaling cues, contributing to the regulation of critical cellular functions.

P35222

Predicted band size: 85 kDa; Observed band size: 85 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3103312

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat, Hamster

WB: 1/500-1/1000;IHC: 1/50-1/100;IP: 1/20

• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using beta Catenin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using beta Catenin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human beta Catenin