ATP1B1 Antibody (YA2084)

ATP1B1 Antibody (YA2084) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP1B1.

The ATP1B1 protein serves as the non-catalytic component of the active enzyme that catalyzes the hydrolysis of ATP, facilitating the exchange of Na(+) and K(+) ions across the plasma membrane. Its regulatory role is manifested through the assembly of alpha/beta heterodimers, controlling the number of sodium pumps transported to the plasma membrane. Beyond its involvement in ion transport, ATP1B1 plays a vital role in innate immunity by amplifying virus-triggered induction of interferons (IFNs) and interferon-stimulated genes (ISGs). This immune-modulatory function involves the enhancement of TRAF3 and TRAF6 ubiquitination, as well as TAK1 and TBK1 phosphorylation. Additionally, ATP1B1 is implicated in cell adhesion and the establishment of epithelial cell polarity, underscoring its multifaceted contributions to cellular processes beyond ion homeostasis.

P05026

Predicted band size: 35 kDa; Observed band size: 40-50 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104448

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100

• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using ATP1B1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using ATP1B1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

Liquid

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human ATP1B1