ATM Antibody (YA597)

ATM Antibody (YA597) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATM.

ATM is a serine/threonine protein kinase that activates checkpoint signaling in response to double-strand breaks (DSBs), apoptosis, and genotoxic stresses such as ionizing radiation, functioning as a DNA damage sensor. It recognizes the substrate consensus sequence [ST]-Q and phosphorylates histone variant H2AX at Ser-139 at DSBs, thereby regulating the DNA damage response mechanism (By similarity). Additionally, ATM plays a role in pre-B cell allelic exclusion by repositioning immunoglobulin heavy chain alleles to enforce clonality. It is involved in signal transduction, cell cycle control, and may act as a tumor suppressor. ATM phosphorylates various substrates, including DYRK2, CHEK2, p53/TP53, BRCA1, etc., modulating their functions. Under genotoxic stress, ATM prevents ubiquitin-mediated degradation of DYRK2 and enhances the DNA damage response by phosphorylating ATF2 and ERCC6. Furthermore, ATM participates in pexophagy by phosphorylating PEX5, promoting its ubiquitination and degradation.

Free

Q13315

Predicted band size: 351 kDa; Observed band size: 370 kDa

Protein A affinity purified.

Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome By Similarity.

Non-conjugated

Unmodified

AB_3102056

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:5,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:50-1:1,000

• 
  Immunocytochemistry analysis of Hela cells labeling ATM with ATM Antibody (HY-P80023)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATM Antibody (HY-P80023) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling ATM with ATM Antibody (HY-P80023)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATM Antibody (HY-P80023) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Rat colon tissue using ATM Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80023, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat colon tissue using ATM Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80023, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human ATM.AA range:1951-2000.