APAF1 Antibody (YA1481)

APAF1 Antibody (YA1481) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to APAF1.

APAF1 Protein, as an essential component of the apoptosome, orchestrates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), initiating the caspase-3 activation cascade and ultimately leading to apoptosis. The oligomeric form of APAF1 forms a heptameric ring, known as the apoptosome, in the presence of cytochrome c and dATP. Within this complex, APAF1 and pro-caspase-9 interact via their NH2-terminal CARD domains, leading to the release of mature caspase-9. Concurrently, pro-caspase-3 is recruited into the APAF1-pro-caspase-9 complex through interaction with pro-caspase-9, facilitating the activation of caspase-3. Notably, isoform 6 of APAF1 exhibits reduced effectiveness in inducing apoptosis. Additionally, APAF1 interacts with APIP, NAIP/BIRC1, and CIAO2A, contributing to its multifaceted role in the regulation of apoptotic pathways.

O14727

Predicted band size: 142 kDa; Observed band size: 135 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3103842

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200

• 
  Immunohistochemical analysis of paraffin-embedded mouse heart tissue using APAF1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse heart tissue using APAF1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human APAF1