ALKBH5 Antibody (YA3429)

ALKBH5 Antibody is an unconjugated, approximately 44 kDa, rabbit-derived, anti-ALKBH5 monoclonal antibody. ALKBH5 Antibody can be used for: WB/IHC-P expriments in human,mouse,rat background without labeling.

Q6P6C2

Predicted band size: 44 kDa;Observed band size: 44 kDa

affinity purified

Nucleus speckle

Non-conjugated

Unmodified

AB_3676580

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal, Recombinant

Rabbit

Human, Mouse, Rat

WB: 1:1,000;IHC-P: 1:1,000-1:2,000;

• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using ALKBH5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using ALKBH5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

HEK-293 cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, F9 cell lysate, PC-12 cell lysate, rat testis tissue lysate, mouse testis tissue

Liquid

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein within human ALKBH5 aa 51-350 / 394.