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  4. ALKBH5 Antibody (YA3429)

ALKBH5 Antibody (YA3429)

Cat. No.: HY-P83693
COA User Guide for Antibodies Technical Support

ALKBH5 Antibody is an unconjugated, approximately 44 kDa, rabbit-derived, anti-ALKBH5 monoclonal antibody. ALKBH5 Antibody can be used for: WB/IHC-P expriments in human,mouse,rat background without labeling.

For research use only. We do not sell to patients.

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10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

ALKBH5 Antibody is an unconjugated, approximately 44 kDa, rabbit-derived, anti-ALKBH5 monoclonal antibody. ALKBH5 Antibody can be used for: WB/IHC-P expriments in human,mouse,rat background without labeling.

Background

ALKBH5 is a dioxygenase that specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification in messenger RNA (mRNA) of higher eukaryotes (by similar). It mediates RNA demethylation through an oxidative mechanism requiring molecular oxygen, alpha-ketoglutarate, and iron as cofactors, thereby regulating mRNA processing, translation, and nuclear export (by similar). ALKBH5 can also demethylate N(6)-methyladenosine in single-stranded DNA in vitro (by similar). During spermatogenesis, ALKBH5 supports the late meiotic and haploid phases by mediating m6A demethylation in spermatocytes and round spermatids, ensuring proper splicing and generation of longer 3’-UTR mRNAs in male germ cells (by similar). Additionally, ALKBH5 participates in paraspeckle assembly—a nuclear membraneless organelle—via liquid-liquid phase separation, coupled with m6A demethylation of transcripts like NEAT1 non-coding RNA (by similar). In immune regulation, ALKBH5 acts as a negative regulator by inhibiting gamma-delta T-cell proliferation through demethylation of JAG1 and NOTCH2 transcripts, and suppressing regulatory T-cell (Treg) recruitment via demethylation and destabilization of CCL28 mRNAs (by similar).

Gene ID
SwissProt ID
Synonyms
ABH5,AlkB,OFOXD,OFOXD1,RNA demethylase ALKBH5 antibody
Molecular Weight

Predicted band size: 44 kDa;Observed band size: 44 kDa

Purity

affinity purified

Subcellular Location

Nucleus speckle

Conjugation

Non-conjugated

Modification

Unmodified

RRID
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Monoclonal, Recombinant

Host

Rabbit

Reactivity

Human, Mouse, Rat

Dilution Ratio

WB: 1:1,000;IHC-P: 1:1,000-1:2,000;

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using ALKBH5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using ALKBH5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application

WB, IHC-P

Positive Control

HEK-293 cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, F9 cell lysate, PC-12 cell lysate, rat testis tissue lysate, mou

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

Recombinant protein within human ALKBH5 aa 51-350 / 394.

Database
Documentation

ALKBH5 Antibody (YA3429) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ALKBH5 Antibody (YA3429)
Cat. No.:
HY-P83693
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