ALIX Antibody (YA6329)

ALIX Antibody (YA6329) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALIX. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

ALIX is a multifunctional protein involved in endocytosis, multivesicular body (MVB) biogenesis, membrane repair, cytokinesis, apoptosis, and tight junction maintenance. As a Class E VPS protein, it facilitates cargo sorting into intralumenal vesicles (ILVs) within MVBs by interacting with ESCRT complexes (ESCRT-O, -I, -II, -III) and binding lysobisphosphatidic acid (LBPA). It acts as an adapter for ESCRT-III proteins (e.g., CHMP4) and is essential for cytokinesis completion. ALIX regulates exosome biogenesis with SDC1/4 and SDCBP and may modulate apoptosis and cell proliferation. By interacting with F-actin, PARD3, and TJP1, it ensures proper actomyosin-tight junction assembly in epithelial polarity (By similarity). Additionally, ALIX supports HIV-1 budding by functionally replacing TSG101 via CHMP4B interaction, highlighting its role in viral release and topologically similar membrane fission events.

Q8WUM4

Predicted band size: 96 kDa; Observed band size: 96 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-1000; WB: 1:1000-5000; ICC/IF: 1:200-1000; ELISA: 1:5000-20000; IP: 1:50-200

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG