AKT1 Antibody (YA834)

AKT1 Antibody (YA834) is a non-conjugated and Mouse origined monoclonal antibody about 56 kDa, targeting to AKT1. It can be used for WB,ICC,IHC-P,FC assays with tag free, in the background of Human.

Free

P31749

Predicted band size: 56 kDa; Observed band size: 56 kDa

Protein A affinity purified.

Nucleus, cytoplasm, cell membrane.

Non-conjugated

Unmodified

AB_3102051

Signal Transduction

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:2,000 ;IHC-P: 1:100-1:200

• 
  Immunocytochemistry analysis of Hela cells labeling AKT1 with Cleaved AKT1 Antibody (HY-P80007) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with AKT1 Antibody (HY-P80007) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling AKT1 with AKT1 Antibody (HY-P80007) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with AKT1 Antibody (HY-P80007)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-P

Liquid

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Endogenous

Synthetic peptide corresponding to Human AKT1.AA range:1-80.