ADAR1 Antibody (YA1658)

ADAR1 Antibody (YA1658) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1658), targeting ADAR1, with a predicted molecular weight of 136 kDa (observed band size: 150 kDa). ADAR1 Antibody (YA1658) can be used for WB, IHC-P, ICC/IF, FC experiment in human background.

ADAR catalyzes adenosine-to-inosine (A-to-I) editing in double-stranded RNA (dsRNA), influencing gene expression through multiple mechanisms: altering protein coding sequences (as inosine is read as guanosine), modulating pre-mRNA splicing, regulating RNA stability, and affecting RNA-structured processes like microRNA biogenesis. It edits both viral (e.g., HCV, HIV-1) and cellular RNAs (e.g., BLCAP, glutamate/serotonin receptors) via site-specific (e.g., GRIA2 R/G site) or hyper-editing modes. ADAR exhibits dual roles in viral infections—pro-viral (enhancing HDV, HIV-1 replication) or antiviral (inhibiting HCV)—through editing-dependent (e.g., HDV amber/W site editing to produce L-HDAg) or independent mechanisms (e.g., suppressing EIF2AK2/PKR for HIV-1). Notably, excessive ADAR activity inhibits HDV replication.

P55265

Predicted band size: 136 kDa; Observed band size: 150 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3103145

Microbiology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;FC: 1/50-1/100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , HEK293 (lane 3(20μg) and HepG2(lane 4(20μg) using ADAR1 (HY-P81913) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling ADAR1 with ADAR1 antibody (HY-P81913) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADAR1 antibody (HY-P81913) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Flow cytometric analysis of 1X10^6 Hela cells labeling ADAR1 Antibody (YA1658) (HY-P81913, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, IHC-P, ICC/IF, FC

Liquid

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human ADAR1 aa200-250.