12 Lipoxygenase Antibody (YA2038)

12 Lipoxygenase Antibody (YA2038) is a non-conjugated IgG antibody, targeting 12 Lipoxygenase, with a predicted molecular weight of 76 kDa (observed band size: 70 kDa). 12 Lipoxygenase Antibody (YA2038) can be used for WB experiment in human, rat background.

12 Lipoxygenase catalyzes the regio- and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids, generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species; it mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE, and through the production of bioactive lipids like (12S)-HPETE, it regulates different biological processes including platelet activation; it can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA, resulting in the formation of (13S,14S)-epoxy-DHA; furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, which actively down-regulate the immune response and have anti-aggregation properties with platelets; an additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both of which are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites; it can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/(13S-HPODE) (By similarity); due to its role in regulating both the expression of vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic; it is important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells.

P18054

Predicted band size: 76 kDa; Observed band size: 70 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104404

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1/500-1/1000

WB

Liquid

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human LOX12