2. Immunology/Inflammation
  3. CD47
  4. Anti-CD47 Antibody (B6.H12)

Anti-CD47 Antibody (B6.H12) 

Cat. No.: HY-P990858 Purity: 95.00%
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Anti-CD47 Antibody (B6.H12) is a kind of mouse IgG1 κ chimeric antibody, targeting to human CD47. Anti-CD47 Antibody (B6.H12) blocks CD47 interactions with SIRPα. Anti-CD47 Antibody (B6.H12) inhibits cell proliferation, cell migration and invasion. Anti-CD47 Antibody (B6.H12) increases macrophage phagocytosis. Anti-CD47 Antibody (B6.H12) shows potent anti-tumor effect in various tumor models, such as osteosarcoma.

For research use only. We do not sell to patients.

Anti-CD47 Antibody (B6.H12)

Anti-CD47 Antibody (B6.H12) Chemical Structure

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Description

Anti-CD47 Antibody (B6.H12) is a kind of mouse IgG1 κ chimeric antibody, targeting to human CD47. Anti-CD47 Antibody (B6.H12) blocks CD47 interactions with SIRPα. Anti-CD47 Antibody (B6.H12) inhibits cell proliferation, cell migration and invasion. Anti-CD47 Antibody (B6.H12) increases macrophage phagocytosis. Anti-CD47 Antibody (B6.H12) shows potent anti-tumor effect in various tumor models, such as osteosarcoma[1][2][3].

Isotype

Mouse IgG1 kappa
Species Reactivity

Human
IC50 & Target

CD47
In Vitro

Anti-CD47 Antibody (B6.H12) (10 μg/mL, 2 h) increases macrophage phagocytosis of HepG2 and H3B cells[1].
Anti-CD47 Antibody (B6.H12) (1.1-10 μg/mL, 24-72 h) is not cytotoxic to human hepatocellular carcinoma cells and normal hepatocytes[1].
Anti-CD47 Antibody (B6.H12) (10 μg/mL, 2 h) significantly decreases the proliferation of IOMM-Lee cells[2].
Anti-CD47 Antibody (B6.H12) (10 μg/mL, 24 h) significantly attenuates cell migration and invasion ability in IOMM-Lee cells[2].
Anti-CD47 Antibody (B6.H12) (10 μg/mL, 2 h) induces macrophage-mediated phagocytosis in Meningioma cells[2].
Anti-CD47 Antibody (B6.H12) (100 μg/mL, 5 days) significantly decreases invasion in osteosarcoma cells[3].
Anti-CD47 Antibody (B6.H12) (10 μg/mL, 2 h) increases macrophage phagocytosis of osteosarcoma tumor cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-CD47 Antibody (B6.H12) Related Antibodies
In Vivo

Anti-CD47 Antibody (B6.H12) (400 μg, i.p, twice a week) shows potent anti-tumor effect in Heterotopic and orthotopic tumor mice models[1].
Anti-CD47 Antibody (B6.H12) (100 μg, i.p, daily for 5 weeks) suppresses tumor growth in IOMM-Lee tumor mice models[2].
Anti-CD47 Antibody (B6.H12) (100 μg, i.p, three times weekly) inhibits tumor progression in osteosarcoma mice models[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Heterotopic/orthotopic tumor mice models[1]
Dosage: 400 μg
Administration: Intraperitoneally injection, twice a week
Result: Inhibited tumor growth.
Showed overall high body masses.
Increased intra-tumor migration of macrophages in Heterotopic tumor models.
Animal Model: Nude mice were injected by IOMM-Lee cells[2]
Dosage: 100 μg
Administration: Intraperitoneally injection, daily for 5 weeks
Result: Inhibited tumor growth and exhibited significantly smaller tumor volume.
Animal Model: Osteosarcoma mice models[3]
Dosage: 100 μg
Administration: Intraperitoneally injection, three times weekly
Result: Showed low mean tibial weight and incidence of spontaneous metastasis.
Gene ID

16423  [NCBI]

Accession
Application

ELISA, FACS, Functional assay, Research in vivo
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-CD47 Antibody (B6.H12)]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Anti-CD47 Antibody (B6.H12) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-CD47 Antibody (B6.H12)CD47Cluster of Differentiation 47MER6OA3SIRPαProliferationMacrophageCancerOsteosarcomaIOMM-LeeInhibitorinhibitorinhibit

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