2. Immunology/Inflammation
  3. NOD-like Receptor (NLR)
  4. AMS-17

AMS-17 is a potent NLRP3 inhibitor, inhibiting microglia activation in vitro and in vivo. AMS-17 also inhibits cytokines such as caspase-1, TNF-α, IL-1β and inducible nitric oxide synthase (iNOS) in N9 cells. AMS-17 can be used for researching inflammation-associated neurological disorders, such as vascular dementia (VaD).

For research use only. We do not sell to patients.

AMS-17

AMS-17 Chemical Structure

CAS No. : 3019885-78-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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Based on 1 publication(s) in Google Scholar

AMS-17 Related Antibodies

Top Publications Citing Use of Products

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NLRP3 NLRP1 NOD1 NOD2

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  • Purity & Documentation

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Description

AMS-17 is a potent NLRP3 inhibitor, inhibiting microglia activation in vitro and in vivo. AMS-17 also inhibits cytokines such as caspase-1, TNF-α, IL-1β and inducible nitric oxide synthase (iNOS) in N9 cells. AMS-17 can be used for researching inflammation-associated neurological disorders, such as vascular dementia (VaD)[1][2].

IC50 & Target

NLRP3

 

In Vivo

AMS-17 (20 mg/kg; i.p; for 5 days) improves cognitive function, enhanced blood-brain barrier (BBB) integrity, and promotes remyelination in VaD mice. AMS-17 reduces neurodegeneration and decreases the expression of NLRP3 and its associated proteins, Apoptosis-associated speck-like protein (ASC), and cleaved caspase-1 in the brain. AMS-17 also lowers pro-inflammatory TNF-α and IL-1β levels, while increasing the anti-inflammatory IL-4 level in the blood[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 10-month-old male C57BL/6J mice bearing bilateral common carotid artery stenosis (BCAS) surgery[2].
Dosage: 20 mg/kg
Administration: i.p; daily; 5 consecutive days
Result: Improved Cognitive Function: Rescued cognitive deficits in VaD mice.
Protected Brain Structure: Enhanced blood-brain barrier (BBB) integrity, promoted remyelination, and reduced neurodegeneration.
Inhibited NLRP3 Inflammasome Activation: Significantly decreased expression of NLRP3, ASC, and cleaved caspase-1 in the mice brain.
Modulated Inflammatory Cytokines: Reduced systemic pro-inflammatory cytokine levels (TNF-α, IL-1β) while elevating the anti-inflammatory cytokine IL-4 in the mice blood.
Molecular Weight

386.35

Formula

C15H13F3N4O3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1N(CCCN1S(=O)(C2=CC=C(C=C2)C(F)(F)F)=O)C3=CN=CN=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (258.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5883 mL 12.9416 mL 25.8833 mL
5 mM 0.5177 mL 2.5883 mL 5.1767 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (12.94 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5883 mL 12.9416 mL 25.8833 mL 64.7082 mL
5 mM 0.5177 mL 2.5883 mL 5.1767 mL 12.9416 mL
10 mM 0.2588 mL 1.2942 mL 2.5883 mL 6.4708 mL
15 mM 0.1726 mL 0.8628 mL 1.7256 mL 4.3139 mL
20 mM 0.1294 mL 0.6471 mL 1.2942 mL 3.2354 mL
25 mM 0.1035 mL 0.5177 mL 1.0353 mL 2.5883 mL
30 mM 0.0863 mL 0.4314 mL 0.8628 mL 2.1569 mL
40 mM 0.0647 mL 0.3235 mL 0.6471 mL 1.6177 mL
50 mM 0.0518 mL 0.2588 mL 0.5177 mL 1.2942 mL
60 mM 0.0431 mL 0.2157 mL 0.4314 mL 1.0785 mL
80 mM 0.0324 mL 0.1618 mL 0.3235 mL 0.8089 mL
100 mM 0.0259 mL 0.1294 mL 0.2588 mL 0.6471 mL
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AMS-17 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AMS-173019885-78-7AMS17AMS 17NOD-like Receptor (NLR)Anti-inflammatoryneuroprotectivemicrogliacytokinesN9Inhibitorinhibitorinhibit

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