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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-W273413
    4-Hydrazino-7-nitro-benzofurazan hydrazine adduct
    		99.83%
    4-Hydrazino-7-nitro-benzofurazan hydrazine adduct is a fluorescent reagent for protein labeling.
    4-Hydrazino-7-nitro-benzofurazan hydrazine adduct
  • HY-124298
    Calcein Blue AM
    		99.00%
    Calcein Blue AM is a cellular dye agent.
    Calcein Blue AM
  • HY-D1255
    Sel-green
    		99.98%
    Sel-green, a selective selenol fluorescent probe, is applied to quantify the Sec content in the selenoenzyme thioredoxin reductase and image endogenous Sec in live HepG2 cells.
    Sel-green
  • HY-141576
    C6-NBD Sphinganine
    		99.0%
    C6-NBD Sphinganine is a sphinganine analog and can be used as fluorescent dye for labeling fatty acid.
    C6-NBD Sphinganine
  • HY-15935B
    X-Gluc cyclohexanamine
    		99.96%
    X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).
    X-Gluc cyclohexanamine
  • HY-D0054
    9-(2-Carboxy-2-cyanovinyl)julolidine
    		99.52%
    9-(2-Carboxy-2-cyanovinyl)julolidine is a fluorescent molecular rotor. 9-(2-Carboxy-2-cyanovinyl)julolidine can be used to bind to IgG and Fab, and preparing antibodies.
    9-(2-Carboxy-2-cyanovinyl)julolidine
  • HY-151644
    Fluorescein hydrazide
    		98.90%
    Fluorescein hydrazide is a sensitive fluorescent probe for Hg2+ and Co2+ that induces obvious color changes and fluorescence changes. Fluorescein hydrazide can be prepared by the reaction of fluorescein and hydrazine. Fluorescein hydrazide exhibits an excitation wavelength of 508 nM and an emission wavelength of 531 nM[2][3].
    Fluorescein hydrazide
  • HY-P4419A
    Boc-Asp(OBzl)-Pro-Arg-AMC acetate
    		99.24%
    Boc-Asp(OBzl)-Pro-Arg-AMC acetate is a fluorogenic substrate for the determination of protease activity. Boc-Asp(OBzl)-Pro-Arg-AMC acetate undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal.
    Boc-Asp(OBzl)-Pro-Arg-AMC acetate
  • HY-N8407
    Carminic acid
    Carminic acid is a widely used and orally active natural red pigment that can be used in industries such as food, cosmetics, and pharmaceuticals. In addition, carminic acid has anti-inflammatory and antioxidant activities.
    Carminic acid
  • HY-118907
    Methyl Orange
    		99.85%
    Methyl Orange is a soluble azo dye commonly used as an acid-base indicator and for staining cells and tissue sections, as well as for dyeing textiles. Methyl Orange appears red at a pH of 3.1 and changes to bright yellow as the pH increases to 4.4. Methyl Orange (500 mg/L) exhibits cytotoxicity and can cause DNA damage.
    Methyl Orange
  • HY-139646
    KMG-104
    		99.12%
    KMG-104 is a highly selective fluorescent Mg2+ probe. KMG-104 has been used widely and revealed Mg2+ mobilization in cytoplasm in various types of cells.
    KMG-104
  • HY-D1832
    Vari Fluor 532 TSA (200×)
    Vari Fluor 532 TSA (200×) (VF 532 Tyramide) is a fluorescent probe belonging to the Vari Fluor TSA series. Vari Fluor TSA series fluorescent probes can be used for high-density in situ labeling of target antigens using horseradish peroxidase (HRP) assays, which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 532 TSA (200×)
  • HY-130022
    HKPerox-1
    		99.35%
    HKPerox-1 is a highly sensitive green fluorescent probe for the specific detection of H2O2 in living cells with a maximum excitation wavelength and emission wavelength of 520 nm and 543 nm, respectively.
    HKPerox-1
  • HY-D0797
    Azomethine-H monosodium
    Azomethine-H monosodium is a colour-forming reagent. Azomethine-H monosodium is also a reagent for boron determinations.
    Azomethine-H monosodium
  • HY-D1098
    SYBR Green II
    SYBR Green II is a fluorescent nucleic acid dye that mainly binds single-stranded nucleotides. SYBR Green II is sensitive to oligonucleotides or larger nucleic acid polymers in a variety of cells and gels. SYBR Green II can be used to study cell structure, membrane integrity or function, and cell cycle distribution. Wavelength 484/515 nm.
    SYBR Green II
  • HY-D0983
    Trypan red
    Trypan red is a vital stain.
    Trypan red
  • HY-D1321A
    Cy 5 amine TFA
    		99.83%
    Cy 5 amine (Cyanine5 amine) TFA is a fluorescent dye. Cy 5 amine TFA can be used in the preparation of Cy5.5-labeled compound or polymers, which can be used for imaging cellular process and trafficking.
    Cy 5 amine TFA
  • HY-D1588
    BODIPY-X-Alkyne
    BODIPY-X-Alkyne is an alkyne-modified BODIPY derivative (Ex=526 nm, Em=540 nm). BODIPY-X-Alkyne can react with azides via a copper-catalyzed click reaction.
    BODIPY-X-Alkyne
  • HY-P3949
    Fluorescent Substrate for Glu-Specific Proteases
    		98.17%
    Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate.
    Fluorescent Substrate for Glu-Specific Proteases
  • HY-125815
    Reactive Blue 4
    Reactive Blue 4 is an anthraquinone dye used extensively in the textile industry. Reactive Blue 4 is a single colorimetric chemosensor for sequential determination of multiple analytes with different optical responses in aqueous media.
    Reactive Blue 4
Cat. No. Product Name / Synonyms Application Reactivity