2. Signaling Pathways
  3. Others
  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-154834
    BHQ-1 NHS
    BHQ-1 NHS directly labeled by fluorescently labeled nucleotide.
    BHQ-1 NHS
  • HY-110334
    FFN 206 dihydrochloride
    		98.57%
    FFN 206 dihydrochloride, a fluorescent probe, is used as an excellent Vesicular Monoamine Transporter 2 (VMAT2) substrate with an apparent Km of 1.16 μM. FFN 206 dihydrochloride is capable of detecting VMAT2 activity in intact cells using fluorescence microscopy, with subcellular localization to VMAT2-expressing acidic compartments without apparent labeling of other organelles.
    FFN 206 dihydrochloride
  • HY-W038786
    2,4,6-Trimethylphenol
    		99.98%
    2,4,6-Trimethylphenol is a probe compound shown to react mainly with organic matter (3DOM*). 2,4,6-Trimethylphenol is rapidly oxidized by singlet oxygen in aqueous solution.
    2,4,6-Trimethylphenol
  • HY-15562
    HOE 32021
    		99.48%
    HOE 32021 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    HOE 32021
  • HY-D1809
    Vari Fluor 405-Streptavidin
    Vari Fluor 405-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=405 nm/431 nm.
    Vari Fluor 405-Streptavidin
  • HY-D0022A
    6-Aminofluorescein
    		98.84%
    6-Aminofluorescein (6-AF) is a fluorescent labeling reagent. 6-Aminofluorescein can be used to label fullerene-based liposome nanostructures termed ‘buckysomes′ (Ex/Em = 490/515 nm).
    6-Aminofluorescein
  • HY-W027773
    7-Methoxy-4-(trifluoromethyl)coumarin
    		≥98.0%
    7-Methoxy-4-(trifluoromethyl)coumarin (MFC) is a fluorescent substrate for cytochrome P450 and can quantify CYP activity.
    7-Methoxy-4-(trifluoromethyl)coumarin
  • HY-D1306
    TAMRA azide, 6-isomer
    TAMRA azide, 6-isomer is an isomer of TAMRA, a xanthene dye with orange emission.
    TAMRA azide, 6-isomer
  • HY-D0830
    Calcein (tetraethyl ester)
    		99.52%
    Calcein tetraethyl ester is a fluorescent dye, is used in biology as it can be transported through the cellular membrane into live cells, which makes it useful for testing of cell viability and for short-term labeling of cells.
    Calcein (tetraethyl ester)
  • HY-D1422
    ER Thermo Yellow
    ER Thermo Yellow is an endoplasmic reticulum-targeted temperature-visible fluorescent probe with high sensitivity (3.9%/°C).
    ER Thermo Yellow
  • HY-W027544
    MCA
    		98.87%
    MCA (7-Methoxycoumarin-4-acetic acid) is a Coumarin (HY-N0709) derivative. MCA quantitates platelet-activating factor (PAF) by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities.
    MCA
  • HY-126220A
    KMG-301AM TFA
    KMG-301AM TFA is the acetoxy methyl esterified form of KMG-301. KMG-301AM TFA successfully accumulates in mitochondria and then it is hydrolyzed to KMG-301. KMG-301 is an Mg2+-selective fluorescent probe functional in mitochondria in intact cells. Since the mitochondrial membrane is impermeable to KMG-301, it is not released upon depolarization of the mitochondrial membrane potential. KMG-301 can indicate changes in mitochondrial Mg2+ concentration and shows Mg2+ transport across the mitochondrial membrane in the early phases of a cellular model.
    KMG-301AM TFA
  • HY-P2500
    (Arg)9, FAM-labeled
    		99.83%
    (Arg)9, FAM-labeled, a cell-penetrating peptide (CPP), is a nona-arginine (ARG) with FAM label. CPPs have emerged as powerful tools for delivering bioactive cargoes into the cytosol of intact cells.
    (Arg)9, FAM-labeled
  • HY-D1464
    CH1055
    CH1055 is a near-infrared II (NIR-II) fluorescent dye that can be used as a fluorescent probe for in vivo imaging. CH1055 can be conjugated with antibodies or ligands for specific imaging (for example the tumor imaging).
    CH1055
  • HY-124298
    Calcein Blue AM
    		99.00%
    Calcein Blue AM is a cellular dye agent.
    Calcein Blue AM
  • HY-P4426A
    Lys-Pro-AMC diTFA
    Lys-Pro-AMC diTFA is a fluorogenic substrate for the detection and measurement of the activity of specific enzymes.
    Lys-Pro-AMC diTFA
  • HY-D2465
    CY5-Dextran (MW60-80k)
    CY5-Dextran is a fluorescent dye CY5 (HY-D0821)- labeled Dextran (HY-112624). CY5 exhibits a maximal excitation wavelength of 633 nm, and a maximal emission wavelength of 670 nm. Dextran-CY5 can be used to visualize the specific subcellular distribution of dextran at the nanoscale.
    CY5-Dextran (MW60-80k)
  • HY-D1693
    FBBBE
    		≥99.0%
    FBBBE is used to detect the production of H2O2 by cells. FBBBE can be triggered by intracellular H2O2 and converted to fluorescein, resulting in an increase in intracellular fluorescence (Ex=4480 nm, Em=512 nm).
    FBBBE
  • HY-151644
    Fluorescein hydrazide
    		98.90%
    Fluorescein hydrazide is a sensitive fluorescent probe for Hg2+ and Co2+ that induces obvious color changes and fluorescence changes. Fluorescein hydrazide can be prepared by the reaction of fluorescein and hydrazine. Fluorescein hydrazide exhibits an excitation wavelength of 508 nM and an emission wavelength of 531 nM[2][3].
    Fluorescein hydrazide
  • HY-P2208
    Z-IETD-AFC
    		99.78%
    Z-IETD-AFC, a specific fluorescence substrate, can be used to determine the caspase-8 catalytic activity.
    Z-IETD-AFC
Cat. No. Product Name / Synonyms Application Reactivity