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  3. Fluorescent Dye
  4. Z-IETD-AFC

Z-IETD-AFC, a specific fluorescence substrate, can be used to determine the caspase-8 catalytic activity.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Z-IETD-AFC Chemical Structure

Z-IETD-AFC Chemical Structure

CAS No. : 219138-02-0

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Based on 1 publication(s) in Google Scholar

Z-IETD-AFC Related Antibodies

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Description

Z-IETD-AFC, a specific fluorescence substrate, can be used to determine the caspase-8 catalytic activity[1].

Molecular Weight

821.75

Formula

C37H42F3N5O13
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

500
Excitation (Ex)

380
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (121.69 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2169 mL 6.0846 mL 12.1692 mL
5 mM 0.2434 mL 1.2169 mL 2.4338 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2169 mL 6.0846 mL 12.1692 mL 30.4229 mL
5 mM 0.2434 mL 1.2169 mL 2.4338 mL 6.0846 mL
10 mM 0.1217 mL 0.6085 mL 1.2169 mL 3.0423 mL
15 mM 0.0811 mL 0.4056 mL 0.8113 mL 2.0282 mL
20 mM 0.0608 mL 0.3042 mL 0.6085 mL 1.5211 mL
25 mM 0.0487 mL 0.2434 mL 0.4868 mL 1.2169 mL
30 mM 0.0406 mL 0.2028 mL 0.4056 mL 1.0141 mL
40 mM 0.0304 mL 0.1521 mL 0.3042 mL 0.7606 mL
50 mM 0.0243 mL 0.1217 mL 0.2434 mL 0.6085 mL
60 mM 0.0203 mL 0.1014 mL 0.2028 mL 0.5070 mL
80 mM 0.0152 mL 0.0761 mL 0.1521 mL 0.3803 mL
100 mM 0.0122 mL 0.0608 mL 0.1217 mL 0.3042 mL
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Z-IETD-AFC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Z-IETD-AFC219138-02-0Fluorescent Dyefluorescence substratecaspase-8catalytic activityInhibitorinhibitorinhibit

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