2. Signaling Pathways
  3. Others
  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-Y0695
    Amido Black 10B
    		98.19%
    Amido Black 10B (Naphthol Blue Black) is a highly toxic azo dye. Amido Black 10B is genotoxic and mutagenic. Amido Black 10B can cause respiratory problems. Amido Black 10B is used for amino acid dyeing.
    Amido Black 10B
  • HY-112841
    PTZ-343
    		99.58%
    PTZ-343 is a potent enhancer of Luminol (HY-15922). PTZ-343 greatly increases the light output of the peroxidase-catalyzed luminol chemiluminescent oxidation reaction (>80%).
    PTZ-343
  • HY-D1849
    Cy3B NHS ester
    Cy3B NHS ester is a fluorescent dye compound that is commonly used in biomarking and fluorescent labeling experiments, especially for labeling biomolecules containing amino functional groups (amine groups), such as proteins, antibodies or peptides.
    Cy3B NHS ester
  • HY-139337
    SMCy5.5
    SMCy5.5 is an SMCy dye used for lipid droplet labeling with a very high two-photon absorption cross-section. SMCy, unlike the well-known lipid droplet marker Nile Red, has narrow absorption and emission bands in visible light, allowing for multicolor imaging. SMCy is shown to be compatible with fixation, producing high-quality 3D images of lipid droplets in cells and tissues. The high brightness of SMCy5.5 enables efficient tracking and imaging of lipid droplet exchange between adipocytes.
    SMCy5.5
  • HY-145127
    ETZ
    ETZ (C3-CA-DTZ) is a promising luciferase substrate (prosubstrate) activatable in vivo by nonspecific esterase to enhance the brain delivery of the luciferin.
    ETZ
  • HY-131029
    Janelia Fluor® 646, Maleimide
    Janelia Fluor® 646, Maleimide (JF646, Maleimide) is a red fluorescent dye that contains a maleimide group. JF646, Maleimide can be used in cellular imaging. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
    Janelia Fluor® 646, Maleimide
  • HY-123495
    CRANAD-28
    		98.03%
    CRANAD-28 is a robust fluorescent compound for visualization of amyloid beta plaques.
    CRANAD-28
  • HY-D1872
    800CW NHS ester
    800CW NHS ester is a near infrared probe that can be used for labeling lysine residues (λmax=776 nm).
    800CW NHS ester
  • HY-D0075
    DHPDS disodium salt
    DHPDS disodium salt is a pH-sensitive fluorescent dye. DHPDS disodium salt is used to measure intracellular pH (pHi) from the surface fluorescence of the isolated perfused rat liver.
    DHPDS disodium salt
  • HY-P3722A
    Mca-PLAQAV-Dpa-RSSSR-NH2 TFA
    		99.21%
    Mca-PLAQAV-Dpa-RSSSR-NH2 is a fluorescent peptide and as one of fluorescent substrates of TNF-α converting enzyme (TACE): ADAM17, ADAM 9 and ADAM 10. Mca-PLAQAV-Dpa-RSSSR-NH2 is a substrate based on fluorescence resonance energy transfer, and its activity can be determined by the change of fluorescence intensity during pyrolysis.
    Mca-PLAQAV-Dpa-RSSSR-NH2 TFA
  • HY-D0916
    Thiazole orange dimer YOYO 1
    Thiazole orange dimer YOYO 1 is a green fluorescent dye used for DNA staining. It belongs to the monomethine cyanine dye family and is a tetracationic homodimer of oxazole yellow (abbreviated as YO, hence the name YOYO), usually provided as a tetraiodide salt. In aqueous buffer, the free YOYO-1 dye (λmax 458 nm; λmax 564 nm) has a very low fluorescence quantum yield, but after binding to double-stranded DNA through diintercalation, the fluorescence intensity is increased by 3200 times (λmax 489 nm; λmax 509 nm).
    Thiazole orange dimer YOYO 1
  • HY-117468
    Lissamine rhodamine B
    		98.00%
    Lissamine rhodamine B is a red-fluorescent dye, it is a derivative of rhodamine. Lissamine rhodamine B can be used as a fluorescent probe to develop competitive aptamer fluorescence anisotropy/polarization (FA/FP) assays.
    Lissamine rhodamine B
  • HY-101887
    Calcein Blue
    Calcein Blue, a membrane-impermeant fluorescent dye, is a coumarin derivative that contains an iminodiacetic acid structure. Calcein Blue is also a metallofluorochromic indicator.
    Calcein Blue
  • HY-D0142
    Pyrene-1,3,6,8-tetrasulfonic acid tetrasodium
    		≥99.0%
    Pyrene-1,3,6,8-tetrasulfonic acid tetrasodium is a fluorescent dye and pH indicator, also as a ligand of multifunctional metal-organic framework. Pyrene-1,3,6,8-tetrasulfonic acid tetrasodium has been used to detect CO2 release.
    Pyrene-1,3,6,8-tetrasulfonic acid tetrasodium
  • HY-136248
    Cyanine 3 Tyramide
    Cyanine 3 Tyramide (Tyramide-Cy3), an orange fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids.
    Cyanine 3 Tyramide
  • HY-P4417A
    Ac-IEPD-AMC TFA
    Ac-IEPD-AMC TFA is a fluorescent substrate used to measure protease activity. Ac-IEPD-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC fluoresces under UV light irradiation and can emit fluorescent signals.
    Ac-IEPD-AMC TFA
  • HY-W001952
    6-Bromo-2-naphthol
    		99.75%
    6-Bromo-2-naphthol is an RTP (real-time polymerase chain reaction) probe capable of real-time monitoring of PCR reactions and quantification of specific nucleic acid sequences. 6-Bromo-2-naphthol phosphoresces at room temperature. RTP probes are a type of small DNA or RNA sequence labeled with fluorescent dyes and quencher molecules and can be widely used for gene expression analysis, SNP genotyping and pathogen detection.
    6-Bromo-2-naphthol
  • HY-D1050
    DNP-X, SE
    		98.48%
    DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester), the DNP-X acid modified by succinimidyl ester, is an amine-reactive building block for developing a probe, which can be recognized by anti-DNP antibodies. DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester) is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.
    DNP-X, SE
  • HY-D1444
    Propidium monoazide
    		99.30%
    Propidium monoazide is a photoreactive DNA-binding dye that preferentially binds to dsDNA. Propidium monoazide (PMA) prevents DNA from dead bacteria from being amplified during the PCR. PMA-PCR enhanced both the specificity and the sensitivity of PCR. Propidium monoazide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Propidium monoazide
  • HY-N0938
    Safflower yellow
    Safflower yellow is extracted from the flowers of the plant safflower (Carthamus tinctorius) and as the traditional Chinese medicine it has been extensively used for the treatment of cardio cerebrovascular diseases.
    Safflower yellow
Cat. No. Product Name / Synonyms Application Reactivity