Methyl red

Cat. No.: HY-Y0699 Purity: 98.67%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Methyl red is a pH-sensitive acid-base indicator and colorimetric reagent with a pKa of 5.1. Methyl red achieves visual judgment of the titration endpoint through reversible structural changes of protonation (red, pH <4.4) and deprotonation (yellow, pH >6.2). Methyl red is widely used in acid-base titration in chemical analysis and buffer pH monitoring in biochemistry.

For research use only. We do not sell to patients.

Methyl red Chemical Structure

CAS No. : 493-52-7

Size	Stock	Quantity
Free Sample (0.1 - 0.5 mg)	Apply Now
25 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
> 100 g	Get quote

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Other Forms of Methyl red:

Methyl red (Standard) Get quote

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Description

In Vitro

Methyl red is used as an acid-base indicator in titration experiments. When the pH changes from 4.4 to 6.2, the color of the solution changes from red to yellow, which can be used to determine the titration endpoint^[1].
Methyl red can be dissolved in ethanol to make a 0.1% solution. A few drops are added to the solution to be tested. During the titration process, the color change is observed to determine the pH endpoint^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

269.30

Formula

C₁₅H₁₅N₃O₂

CAS No.

493-52-7

Appearance

Solid

Color

Purple to purplish red

SMILES

O=C(C1=C(/N=N/C2=CC=C(N(C)C)C=C2)C=CC=C1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, protect from light

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 70 mg/mL (259.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7133 mL	18.5664 mL	37.1328 mL
5 mM	0.7427 mL	3.7133 mL	7.4266 mL
10 mM	0.3713 mL	1.8566 mL	3.7133 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.67%

Select Batch:

Data Sheet (272 KB) SDS (709 KB)

COA (248 KB) HNMR (265 KB) RP-HPLC (245 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Ikram M, et al. Biodegradation of Azo Dye Methyl Red by Pseudomonas aeruginosa: Optimization of Process Conditions. Int J Environ Res Public Health. 2022 Aug 12;19(16):9962. [Content Brief]

[2]. Kasai, et al. Menthol induces surgical anesthesia and rapid movement in fishes. Open Neuroscience Journal 8 (2014): 1-8.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7133 mL	18.5664 mL	37.1328 mL	92.8319 mL
	5 mM	0.7427 mL	3.7133 mL	7.4266 mL	18.5664 mL
	10 mM	0.3713 mL	1.8566 mL	3.7133 mL	9.2832 mL
	15 mM	0.2476 mL	1.2378 mL	2.4755 mL	6.1888 mL
	20 mM	0.1857 mL	0.9283 mL	1.8566 mL	4.6416 mL
	25 mM	0.1485 mL	0.7427 mL	1.4853 mL	3.7133 mL
	30 mM	0.1238 mL	0.6189 mL	1.2378 mL	3.0944 mL
	40 mM	0.0928 mL	0.4642 mL	0.9283 mL	2.3208 mL
	50 mM	0.0743 mL	0.3713 mL	0.7427 mL	1.8566 mL
	60 mM	0.0619 mL	0.3094 mL	0.6189 mL	1.5472 mL
	80 mM	0.0464 mL	0.2321 mL	0.4642 mL	1.1604 mL
	100 mM	0.0371 mL	0.1857 mL	0.3713 mL	0.9283 mL

Methyl red Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methyl red493-52-7Fluorescent DyepH indicatordyeInhibitorinhibitorinhibit

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