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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-131028
    Janelia Fluor® 646 TFA
    Janelia Fluor® 646 TFA (JF646 TFA), a red fluorogenic fluorescent dye, can be used in the synthesis of Janelia Fluor 646 HaloTag and SNAP-Tag ligands. JF646 TFA is used in live cell imaging experiments. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
    Janelia Fluor® 646 TFA
  • HY-135036
    4-Methylumbelliferyl butyrate
    		99.46%
    4-Methylumbelliferyl butyrate (4-MUB) is a coumarin-based fluorogenic substrate used for the identification of M. catarrhalis C4- esterase. 4-Methylumbelliferyl butyrate can converse to the blue-emissive 4-methylumbelliferone (4-MU; HY-N0187).
    4-Methylumbelliferyl butyrate
  • HY-W110898
    Nile blue chloride
    Nile blue chloride is a highly fluorescent and photostable organic dye. Nile blue chloride and fluorescein isothiocyanate (FITC) can be used to construct a ratiometric pH sensitive probe for tracking the pH of the extracellular fluid between cancer cells in realtime. Nile Blue chloride has the potential for the research of nonlinear optics.
    Nile blue chloride
  • HY-123275A
    H-D-Phe-Pip-Arg-pNA hydrochloride
    		99.40%
    H-D-Phe-Pip-Arg-pNA (S-2238) hydrochloride, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA hydrochloride is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA hydrochloride is sensitive, accurate, and easy to perform.
    H-D-Phe-Pip-Arg-pNA hydrochloride
  • HY-104058
    Oregon-BAPTA Green 1AM
    Oregon-BAPTA Green 1AM (Oregon Green 488 BAPTA 1AM) is a fluorescent Ca2+ indicator that consists of a green fluorescent probe OG 488 and a cell-permeable Ca2+ chelator BAPTA AM.
    Oregon-BAPTA Green 1AM
  • HY-D0789
    ANTS
    		99.09%
    ANTS is a fluorescent dye. ANTS and DPX are encapsulated in liposomes can be an effective approach for measuring membrane leakage.
    ANTS
  • HY-D1148
    HKGreen-4I
    		99.86%
    HKGreen-4I is a highly sensitive green fluorescent probe for the specific detection of ONOO- in living cells with a maximum excitation wavelength and emission wavelength of 520 nm and 543 nm, respectively.
    HKGreen-4I
  • HY-D1250
    Mito-TRFS
    Mito-TRFS, the first off-on probe, is used to image the mitochondrial thioredoxin reductase (TrxR2) in live cells.
    Mito-TRFS
  • HY-133527
    OG 488, SE
    OG 488, SE (Oregon green 488 succinimidyl ester), a fluorescent pH indicator, has many applications in biochemistry and neurosciences.
    OG 488, SE
  • HY-W110927
    Alizarin Red S Indicator (4.3-6.3), IND
    Alizarin red S indicator (C.I. 58005) is a reductively active (quinone-based) anthraquinone dye that forms complexes with metal ions (such as zirconium, calcium) or boric acid to label calcium deposition and perform electrochemical sensing functions. Alizarin Red S undergoes reversible redox reactions (for electrochemical detection) and irreversible chelation (for bone staining). Alizarin Red S is mainly used in bone metabolism research (labeling mineralized tissue), sugar detection (boric acid-sugar competition system) and metal ion sensing (such as zirconium ion detection)， and can be used in osteoporosis and metabolic disease research.
    Alizarin Red S Indicator (4.3-6.3), IND
  • HY-D1605
    BODIPY FL L-Cystine
    		99.88%
    BODIPY FL L-Cystine is a thiol-reactive, green-fluorescent dye. BODIPY FL L-Cystine can be the labeling of membrane proteins, proteins with hydrophobic binding sites, or hydrophobic ligands. (λex=504 nm, λem=511 nm).
    BODIPY FL L-Cystine
  • HY-D0853A
    DiAzKs hydrochloride
    		≥98.0%
    DiAzKs (H-L-Photo-lysine) hydrochloride is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs hydrochloride can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs hydrochloride acts as a UV light-activated photo-crosslinking probe.
    DiAzKs hydrochloride
  • HY-130433
    NBD Sphingosine
    		≥99.0%
    NBD Sphingosine (NBD-Sph), a fluorochrome, is a fluorescence-labeled sphingosine. NBD Sphingosine can be uesd for fluorescence assay for sphingosine kinases.
    NBD Sphingosine
  • HY-D1630
    4-Di-10-ASP
    		≥98.0%
    4-Di-10-ASP is a fluorescent lipophilic tracer (Excitation 485 nm; Emission 620 nm). 4-Di-10-ASP can be used to stain phospholipid membranes in a specific manner.
    4-Di-10-ASP
  • HY-P1883A
    Bacterial Sortase Substrate III, Abz/DNP TFA
    		98.09%
    Bacterial Sortase Substrate III, Abz/DNP TFA is an internally quenched fluorescent peptide substrate. Staphylococcus aureus transpeptidase sortase A (SrtA) reacts with its native substrate Bacterial Sortase Substrate III, Abz/DNP, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this substrate can be monitored at Ex/Em=320 nm/420 nm.
    Bacterial Sortase Substrate III, Abz/DNP TFA
  • HY-B1741
    Proflavine
    		98.78%
    Proflavine, an acridine dye, is a known DNA intercalating agent. Anti-microbial agent. Proflavine behaves as a pore blocker for Kir3.2. Proflavine is a potential lead compound for Kir3.2-associated neurological diseases.
    Proflavine
  • HY-D0933
    Auramine O
    Auramine O is a yellow fluorescent dye. Auramine O is used to detect amyloid fibrils. Auramine O promotes lung malignancy. Auramine O is also used to determine algal cell viability and stain acid-fast bacteria.
    Auramine O
  • HY-NP0147
    Wheat Germ Agglutinin (Fluorescein)
    Wheat Germ Agglutinin (WGA) Fluorescein is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Wheat Germ Agglutinin (WGA) Fluorescein is a biological material or organic compound that can be used in life science research.
    Wheat Germ Agglutinin (Fluorescein)
  • HY-151667
    Biotin-C5-Azide
    		≥98.0%
    Biotin-C5-Azide (DecarboxyBiotin-N3) is a biotin reagent and can be used to prepare biotinylated conjugates. Biotin-C5-Azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Biotin-C5-Azide
  • HY-131131
    5-CFDA-AM
    		99.81%
    5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell.
    5-CFDA-AM
Cat. No. Product Name / Synonyms Application Reactivity