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  4. OG 488, SE

OG 488, SE  (Synonyms: Oregon green 488 succinimidyl ester)

Cat. No.: HY-133527 Purity: 90.88%
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OG 488, SE (Oregon green 488 succinimidyl ester), a fluorescent pH indicator, has many applications in biochemistry and neurosciences.

For research use only. We do not sell to patients.

OG 488, SE

OG 488, SE Chemical Structure

CAS No. : 198139-51-4

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Based on 1 publication(s) in Google Scholar

OG 488, SE Related Antibodies

Top Publications Citing Use of Products

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Description

OG 488, SE (Oregon green 488 succinimidyl ester), a fluorescent pH indicator, has many applications in biochemistry and neurosciences[1][2].

In Vitro

Particle Preparation[1]
1. Dilute 250 µL of a 5.75% polystyrene particle suspension with 0.1 M sodium bicarbonate buffer (pH 8.3) to a total volume of 1.5 mL, achieving a 6-fold dilution. Then, place the mixture in an ice bath and sonicate for 5 minutes.
2. While stirring gently, slowly add 1.5 mL of 300 µM OG 488, SE labeling reagent and an equal amount of 0.1 M sodium bicarbonate buffer (pH 8.3) to the solution.
3. After gently stirring, let the mixture incubate in a dark environment at room temperature for 2 hours.
4. Next, take 15 µL of 40 µM Texas Red and SE labeling reagent dissolved in dimethylformamide and carefully add it to the particle solution under ultrasonic treatment to ensure even dispersion.
5. After incubation, slowly centrifuge the particles at 1000 g for 10 minutes, collect the pellet, and wash it three times with 0.1 M sodium bicarbonate buffer (pH 8.3), finally resuspending the particles in the same buffer for storage. The polystyrene particles are labeled with fluorescent dyes using a chemical labeling method for subsequent experimental applications.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

OG 488, SE Related Antibodies
Molecular Weight

509.37

Formula

C25H13F2NO9
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

512
Excitation (Ex)

488
SMILES

O=C(C1=CC2=C(C3(C4=C(OC5=C3C=C(F)C(O)=C5)C=C(O)C(F)=C4)OC2=O)C=C1)ON6C(CCC6=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (196.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9632 mL 9.8160 mL 19.6321 mL
5 mM 0.3926 mL 1.9632 mL 3.9264 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9632 mL 9.8160 mL 19.6321 mL 49.0802 mL
5 mM 0.3926 mL 1.9632 mL 3.9264 mL 9.8160 mL
10 mM 0.1963 mL 0.9816 mL 1.9632 mL 4.9080 mL
15 mM 0.1309 mL 0.6544 mL 1.3088 mL 3.2720 mL
20 mM 0.0982 mL 0.4908 mL 0.9816 mL 2.4540 mL
25 mM 0.0785 mL 0.3926 mL 0.7853 mL 1.9632 mL
30 mM 0.0654 mL 0.3272 mL 0.6544 mL 1.6360 mL
40 mM 0.0491 mL 0.2454 mL 0.4908 mL 1.2270 mL
50 mM 0.0393 mL 0.1963 mL 0.3926 mL 0.9816 mL
60 mM 0.0327 mL 0.1636 mL 0.3272 mL 0.8180 mL
80 mM 0.0245 mL 0.1227 mL 0.2454 mL 0.6135 mL
100 mM 0.0196 mL 0.0982 mL 0.1963 mL 0.4908 mL
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OG 488, SE Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

OG 488, SE198139-51-4Oregon green 488 succinimidyl esterFluorescent DyeInhibitorinhibitorinhibit

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