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  4. Bacterial Sortase Substrate III, Abz/DNP TFA

Bacterial Sortase Substrate III, Abz/DNP TFA 

Cat. No.: HY-P1883A Purity: 98.09%
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Bacterial Sortase Substrate III, Abz/DNP TFA is an internally quenched fluorescent peptide substrate. Staphylococcus aureus transpeptidase sortase A (SrtA) reacts with its native substrate Bacterial Sortase Substrate III, Abz/DNP, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this substrate can be monitored at Ex/Em=320 nm/420 nm.

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Bacterial Sortase Substrate III, Abz/DNP TFA

Bacterial Sortase Substrate III, Abz/DNP TFA Chemical Structure

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Description

Bacterial Sortase Substrate III, Abz/DNP TFA is an internally quenched fluorescent peptide substrate. Staphylococcus aureus transpeptidase sortase A (SrtA) reacts with its native substrate Bacterial Sortase Substrate III, Abz/DNP, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this substrate can be monitored at Ex/Em=320 nm/420 nm.

Formula

C41H57N11O14.xC2HF3O2
Appearance

Solid

Color

Light yellow to yellow

Sequence

{Abz}-Leu-Pro-Glu-Thr-Gly-{Lys(Dnp)}-NH2
Sequence Shortening

{Abz}-LPETG-{Lys(Dnp)}-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL

*"≥" means soluble, but saturation unknown.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Purity & Documentation
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Bacterial Sortase Substrate III, Abz/DNP TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bacterial Sortase Substrate III, Abz/DNPFluorescent DyeInhibitorinhibitorinhibit

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