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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-W127770
    Pararosaniline hydrochloride
    		98.28%
    Pararosaniline hydrochloride (Basic red 9) is a pH-responsive basic dye, as a biological stain to track certain proteins. The pH of the acidified Pararosaniline hydrochloride reagent has a significant effect on the color and the maximum absorption wavelength (λmax) of the reaction system, with its optimum pH 0.48 and a λmax at 549 nm. Pararosaniline hydrochloride is also a strong modifier of RNA splicing. Pararosaniline hydrochloride has been used in the analysis of SO2 and formaldehyde and staining of bacteria or other organisms. Pararosaniline hydrochloride is extensively used in industries like textile, printing, paper, cosmetic, and leather.
    Pararosaniline hydrochloride
  • HY-122009
    Indican
    		99.88%
    Indican (Indoxyl-β-D-glucoside), a glycoside of indoxyl, is a precursor of the dyesindigo and indirubin. Indican has a major metabolite, indoxyl sulfate (IS). IS, an uremic toxin, is a substrate/inhibitor of organic anion transporter (OAT) 1, OAT 3 and multidrug resistance-associated protein (MRP) 4.
    Indican
  • HY-D0827
    Cy2
    		≥98.0%
    Cyanines are formally compounds with two nitrogen atoms linked by an odd number of methene units. 26 28 The nitrogen atoms are parts of the heterocyclic units (such as indole, benzoxazol, or benzothiazol) . The structures and optical properties of representative cyanine dyes used for in vivo imaging are presented. Cyanines are characterized by long wavelength, tunable absorption and emission, very high extinction coefficient (up to 300,000 M 1 cm 1), good water solubility, and relatively straightforward synthesis.
    Cy2
  • HY-D1256
    Msr-blue
    		99.38%
    Msr-blue is a first turn-on fluorescent probe for methionine sulfoxide reductase with a more than 100-fold fluorescence increment. Msr-blue is used for monitoring the enzyme activity in live cells (λex=340 nm, λem=440 nm).
    Msr-blue
  • HY-129109
    NBD-Pen
    		99.94%
    NBD-Pen is a fluorescence probe. NBD-Pen can be used for the research of detection of lipid-derived radicals.
    NBD-Pen
  • HY-W088068
    Wright's stain
    Wright's stain is a composite cell stain that mainly binds to intracellular nucleic acids, proteins and other components through thiazine dyes (such as methylene blue) and eosin. Wright's stain is pH-dependent (optimal pH 6.4-6.7) and achieves cell morphology resolution by differentially staining the cytoplasm and nucleus. Under alkaline conditions, thiazine dyes bind to nucleic acids to form purple, and acidic eosin binds to cytoplasmic proteins to form red, which can form contrasting cell morphological features. Wright's stain can clearly display the fine structures of blood cells and bone marrow cells (such as nuclear chromatin and granules) and quickly evaluate cell morphological abnormalities.
    Wright's stain
  • HY-139109
    IR-783
    		99.87%
    IR-783 (ADS 780WS) is a near-infrared (NIR) heptamethine cyanine fluorescent probe. IR-783 significantly inhibits tumour growth and induces apoptosis in MDA-MB-231 xenograft model. IR-783 can be used to study breast cancer.
    IR-783
  • HY-126395
    Patent Blue V calcium salt
    		99.99%
    Patent Blue V calcium salt is a triarylmethane dye used for tissue staining and lymphatic tracing, mainly applied by topical injection or eye drops. Patent Blue V calcium salt has affinity for specific tissues (such as corneal endothelium, lymphatic system), and stains the target structure by adsorption or binding, assisting in precise operation during surgery. Patent Blue V calcium salt is mainly used in ophthalmic surgery (such as graft staining for Descemet's membrane endothelial keratoplasty) and lymphatic drainage localization for sentinel lymph node biopsy of tumors.
    Patent Blue V calcium salt
  • HY-D0941
    5-Carboxytetramethylrhodamine
    		99.13%
    5-Carboxytetramethylrhodamine can be used as a fluorescent probe of nucleic acids and proteins. 5-Carboxytetramethylrhodamine displays excitation maxima of 558 nm and an emission maximum of 586 nm.
    5-Carboxytetramethylrhodamine
  • HY-D2189
    IRDye 700DX
    IRDye 700DX (IRDye 700DX NHS ester) is a near-infrared (NIR) phthalocyanine dye with extremely high photostability and fluorescence intensity. IRDye 700DX binds to biomolecules. IRDye 700DX has excellent water solubility, large extinction coefficient, high fluorescence quantum yield, and does not aggregate in high ionic strength buffers. IRDye 700DX can be used as a highly flexible photosensitizer.
    IRDye 700DX
  • HY-D1694
    LysoTracker Yellow HCK 123
    		98.51%
    LysoTracker Yellow HCK 123 is a potent yellow membrane-permeable fluorescent probe. LysoTracker Yellow HCK 123 is a weakly basic amine that selectively accumulates in cellular compartments with low luminal pH.
    LysoTracker Yellow HCK 123
  • HY-D0166
    Neutral Red
    		98.14%
    Neutral Red, a nitrogenous pH-indicator with a pKi of 6.8, is an indicator for the internal acidification of thylakoids. Neutral Red stains lysosomes red.
    Neutral Red
  • HY-D1878
    SPiDER-βGal-1
    		99.39%
    SPiDER-βGal-1 is a potent probe for β-galactosidase activity and suitable for labeling live cells in culture.
    SPiDER-βGal-1
  • HY-130013
    HKYellow-AM (6/12-mixture)
    		99.36%
    HKYellow-AM (6/12-mixture) is a yellow fluorescent probe that can detect ONOO- in living cells and tissues with high selectivity and sensitivity without cytotoxicity.
    HKYellow-AM (6/12-mixture)
  • HY-131013
    GSHtracer
    		98.06%
    GSHtracer is a ratiometric probe for measuring of GSH levels. GSHtracer exhibits Ex/Em from 520/580 nm to 430/510 nm (upon GSH binding) .
    GSHtracer
  • HY-158082B
    TRITC-dextran, MW 40000
    TRITC-dextran, MW 40000 (Tetramethyl rhodamine isothiocyanate glucan, MW 40000) is a fluorescent dye, with the molecular weight of 40 kD. TRITC-dextran MW 40000 exhibits an excitation wavelength of 555 nm. TRITC-dextran MW 40000 is vessel penetrate, which could label blood plasma to visualize the vasculature. TRITC-dextran MW 40000 is utilized in drug delivery for the stability of TRITC over a wide pH range (i.e. pH 2–11) and resistance to photo-bleaching.
    TRITC-dextran, MW 40000
  • HY-D1353
    LipidGreen 2
    		98.94%
    LipidGreen 2 is a second generation small molecule probe for lipid imaging. LipidGreen 2 has a better fluorescence signal compared with the previous LipidGreen, and selectively stains neutral lipids in cells and fat deposits in live zebrafish.
    LipidGreen 2
  • HY-D1568
    Sulfo-Cy7.5 NHS ester
    Sulfo-Cy7.5 NHS ester is a near infrared water soluble hydrophilic dye, also is an NHS ester for the modification of amine groups. Sulfo-Cy7.5 NHS ester contains a trimeth ylene bridge and has a linker arm for its attachment to proteins, peptides, and other molecules. Sulfo-Cy7.5 NHS ester can be used for the research of NIR imaging applications.
    Sulfo-Cy7.5 NHS ester
  • HY-D0076
    4,5-Diaminofluorescein
    		99.34%
    4,5-Diaminofluorescein is a fluorescent detector for nitric oxide (NO) in cells and tissues.
    4,5-Diaminofluorescein
  • HY-131027
    Janelia Fluor® 646, Azide
    		99.87%
    Janelia Fluor 646, Azide (JF646, Azide) is a red fluorogenic fluorescent dye containing a click chemistry group Azide. Janelia Fluor 646, Azide can be used for live-cell imaging experiments. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Janelia Fluor? 646, Azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Janelia Fluor® 646, Azide
Cat. No. Product Name / Synonyms Application Reactivity