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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-129109
    NBD-Pen
    		99.94%
    NBD-Pen is the first fluorescence probe for lipid radicals with high selectivity and sensitivity (λex: 470 nm, λem: 530 nm). NBD-Pen specifically detects lipid derived radicals over other reactive species present in biological systems, including H2O2, ClO-, O2-∙, and ∙OH. NBD-Pen directly detects lipid radicals in living cells by turn-on fluorescence. NBD-Pen decreases inflammation, apoptosis, and oxidative stress markers. NBD-Pen can be studied in various disease models such as hepatic carcinoma.
    NBD-Pen
  • HY-D1539
    Cyanine 5.5 azide
    		98.02%
    Cyanine 5.5 azide (CY 5.5 azide) is a potent fluorescent dye. Cyanine 5.5 azide can label DNA. Cyanine 5.5 azide can be used for NIR live organism imaging. (λex=684 nm, λem=710 nm). Cyanine 5.5 azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Cyanine 5.5 azide
  • HY-D2189
    IRDye 700DX
    IRDye 700DX (IRDye 700DX NHS ester) is a near-infrared (NIR) phthalocyanine dye with extremely high photostability and fluorescence intensity. IRDye 700DX binds to biomolecules. IRDye 700DX has excellent water solubility, large extinction coefficient, high fluorescence quantum yield, and does not aggregate in high ionic strength buffers. IRDye 700DX can be used as a highly flexible photosensitizer.
    IRDye 700DX
  • HY-D1627
    CellTracker CM-DiI
    		≥98.0%
    CellTracker CM-DiI (CM-DiI) is a lipophilic fluorochrome that can be used for tracking the migration of lymphocytes. CellTracker CM-DiI is feasible to detect labeled cells in histological sections (excitation 553 nm; emission 570 nm).
    CellTracker CM-DiI
  • HY-D1878
    SPiDER-βGal-1
    		99.39%
    SPiDER-βGal-1 is a fluorogenic β-galactosidase substrate and suitable for labeling live cells in culture and in living tissues. SPiDER-βGal-1 can generate quinone methide through enzymatic reaction and form covalent bonds with nucleophilic functional groups of proteins near the molecule, so that the fluorescent product is fixed in living cells (Ex/Em = 493, 525 nm/560 nm).
    SPiDER-βGal-1
  • HY-130013
    HKYellow-AM (6/12-mixture)
    		99.36%
    HKYellow-AM (6/12-mixture) is a yellow fluorescent probe that can detect ONOO- in living cells and tissues with high selectivity and sensitivity without cytotoxicity.
    HKYellow-AM (6/12-mixture)
  • HY-111263
    NIAD-4
    		99.11%
    NIAD-4 is a fluorophore for optical imaging of amyloid-β (Aβ) in the central nervous system (CNS) for Alzheimer’s disease (AD). NIAD-4 binds to the same Aβ site with the binding affinity (Ki) of 10 nM.
    NIAD-4
  • HY-D1610
    BODIPY FL C5
    		99.68%
    BODIPY FL C5 is a green fluorescent fatty acid. BODIPY FL C5 can be used as a precursor for the synthesis of various fluorescent phospholipids. BODIPY FL C5 is relatively insensitive to the environment and fluoresces in both water-soluble and lipid environments.
    BODIPY FL C5
  • HY-130025
    HKOCl-3
    		99.51%
    HKOCl-3 is a highly sensitive and selective fluorescent probe for detecting hypochlorous acid.Ex: 490 nm; Em 527 nm.
    HKOCl-3
  • HY-101898
    Indo-1 AM
    Calcium is an important part of the human body, usually in the form of calcium, a large number of bones and teeth of the human body, a small amount of blood and tissues. MCE calcium ion detection probe can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence, in addition, Mag-indo-1/AM and Mag-Fluo-4 AM at a certain concentration (usually 5 mM) can effectively identify intracellular magnesium ions.
    Indo-1 AM
  • HY-D1341
    Coumberone
    		99.95%
    Coumberone is a metabolic fluorogenic probe, and isoform-selective substrate for all AKR1C isoforms. Coumberone can be reduced by all four members of the AKR1C family to its fluorescent alcohol coumberol. Coumberone can be used for the research of AKR1C.
    Coumberone
  • HY-D0203
    Basic green 4
    Basic green 4 (Malachite green) is a cationic dye that is widely used as a bactericide in aquaculture and is also commonly used in laboratories for the determination of phosphates. Basic green 4 exhibits high cytotoxicity and carcinogenicity to mammalian cells, specifically shown by its IC50 values of 2.03 µM for HEp-2 human laryngeal cells and 13.8 µM for Caco-2 human colorectal adenocarcinoma cells, and it can promote the development of liver tumors. The maximum absorbance wavelength is 621 nm.
    Basic green 4
  • HY-D1161
    True Blue
    True Blue (NCI 240899) is a fluorescent dye, as neuronal retrograde tracer (excitation wavelength 395-425 nm, barrier filter 450 nm). True Blue can label neuron and has no effects on neuronal survival.
    True Blue
  • HY-130533
    ReAsH-EDT2
    ReAsH-EDT2 is a red fluorescent dye that marks proteins. ReAsH-EDT2 is a membrane-permeable biarsenical compound that binds covalently to tetracysteine sequences which allows the protein to be imaged. ReAsH-EDT2 can be used for protein localization and trafficking. (λex=530 nm, λem=592 nm).
    ReAsH-EDT2
  • HY-15922A
    Luminol sodium salt
    		99.84%
    Luminol sodium salt is a chemical that exhibits chemiluminescence with pKa values of 6.74 and 15.1. Luminol sodium salt exhibits chemiluminescence (CL) at 425 nm λmax. Luminol sodium salt is commonly used in forensics as a diagnostic tool for the detection of blood stains.
    Luminol sodium salt
  • HY-151813
    NNMT-IN-4
    		99.35%
    NNMT-IN-4 (compound 38) is a selective, uncompetitive and membrane permeability nicotinamide N-methyltransferase (NNMT) inhibitor with IC50 values of 42 and 38 nM in vitro biochemical and cell-based assays, respectively. NNMT-IN-4 shows favorable PK/PD and safety profiles as well as excellent oral bioavailability and pharmaceutical properties. NNMT-IN-4 can be used as a vivo chemical probe of NNMT.
    NNMT-IN-4
  • HY-D0099
    Fluorescein-5-thiosemicarbazide
    Fluorescein-5-thiosemicarbazide is a amine containing fluorescent probe that can be used for labeling saccharides and protein carbonyl derivatives.
    Fluorescein-5-thiosemicarbazide
  • HY-143702
    Fluorescent DOTAP
    Fluorescent DOTAP (NBD-DOTAP) is a cationic lipid which can be used for nucleic acid and protein delivery. Fluorescent DOTAP is labeled with a fluorophore NBD (maximum excitation/emission wavelength ∼463/536 nm). Fluorescent DOTAP can be used for gene delivery systems, drug delivery, as well as cell imaging and nanocarrier tracking. Fluorescent DOTAP is an ideal candidate for both biological and pharmaceutical formulation (e.g. co-delivery of vaccines) research.
    Fluorescent DOTAP
  • HY-124418
    SBI-477
    		≥98.0%
    SBI-477 is a chemical probe that stimulates insulin signaling by deactivating the transcription factor MondoA. SBI-477 can lead to reduced expression of the insulin pathway suppressors thioredoxin-interacting protein (TXNIP) and arrestin domain-containing 4 (ARRDC4). SBI-477 coordinately inhibits triacylglyceride (TAG) synthesis and enhances basal glucose uptake in human skeletal myocytes.
    SBI-477
  • HY-164745
    L-β-Ethynylserine
    		99.98%
    L-β-ethynylserine (βES) is a threonine analog. L-β-ethynylserine is efficiently incorporated into newly synthesized proteins, and allows their selective visualization or enrichment through bioorthogonal ligation to fluorescent dyes or affinity tags, respectively.
    L-β-Ethynylserine
Cat. No. Product Name / Synonyms Application Reactivity