Mafenide Acetate

Name: Mafenide Acetate
Brand: MedChemExpress
SKU: HY-B0614A
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0614A Purity: 99.91%: Data Sheet
COA

SDS
Handling Instructions
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Mafenide Acetate is a potent sulfonamide antimicrobial agent. Mafenide Acetate exhibits antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. Mafenide Acetate also exhibits antifungal activity against filamentous fungi (e.g., Lichtheimia and Aspergillus flavus). Mafenide Acetate can be used in the research of skin grafts on burn wounds, post-traumatic invasive fungal infections, and bacterially contaminated wounds.

For research use only. We do not sell to patients.

Mafenide Acetate Chemical Structure

CAS No. : 13009-99-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Mafenide Acetate:

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Mafenide Acetate (5%) maintains antimicrobial activity with a zone of inhibition >2 mm against Staphylococcus aureus and Pseudomonas aeruginosa in vitro^[1].
Mafenide Acetate (2.5%-7.5%; 1-24 h) exhibits dose-dependent antifungal activity against filamentous fungi (e.g., Lichtheimia spp., Aspergillus flavus)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	Human keratinocytes (HEK-001; ATCC CRL-2404), human dermal fibroblasts (PromoCell), human osteoblasts (PromoCell)
Concentration:	2.5%, 5%, 7.5%
Incubation Time:	0.5 h, 1 h, 3 h, 24 h
Result:	Showed a viability range of 30%-78% (fibroblasts). Showed a viability range of 31%-77% (keratinocytes). Showed a viability range of 22%-81% (osteoblasts).

In Vivo

Mafenide Acetate (2.5% solution; soaked in hydrofiber or gauze dressings, applied topically; group 3: changed every 2 days, group 4: changed twice a day; 14 days) improves skin graft survival on day 7 but causes significant cytotoxicity and graft loss by day 14 in a Pseudomonas aeruginosa-contaminated full-thickness skin graft (FTSG) model in male Wistar albino rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar albino rats (mean weight 200 g) with Pseudomonas aeruginosa-contaminated full-thickness skin graft (FTSG) model (2.5×2.5 cm FTSG harvested from back, wound bed inoculated with 10⁷ CFU/mL Pseudomonas aeruginosa in 0.6 mL buffer solution)^[4]
Dosage:	2.5% Mafenide Acetate solution
Administration:	Topical application via soaked dressings (hydrofiber or gauze); group 3 (hydrofiber + MA): dressing changed every 2 days; group 4 (gauze + MA): dressing changed twice a day; total cycle of 14 days
Result:	Showed a mean graft survival rate of 50.1% in group 3 and 66.3% in group 4. Showed detachment of the dermal-epidermal junction, collagen disorganization, decreased fibroblast number, and decreased capillary count.

Clinical Trial

Molecular Weight

246.28

Formula

C₉H₁₄N₂O₄S

CAS No.

13009-99-9

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C(CN)C=C1)(N)=O.CC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (406.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (406.04 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0604 mL	20.3021 mL	40.6042 mL
5 mM	0.8121 mL	4.0604 mL	8.1208 mL
10 mM	0.4060 mL	2.0302 mL	4.0604 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 70 mg/mL (284.23 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (295 KB) SDS (393 KB)

COA (251 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Ashkan Afshari, et al. The Effective Duration of Antimicrobial Activity of Mafenide Acetate After Reconstitution. J Burn Care Res. 2018 Aug 17;39(5):736-738. [Content Brief]

[2]. Haynes, B.W., Jr., Mafenide acetate in burn treatment. N Engl J Med, 1971. 284(23): p. 1324. [Content Brief]

[3]. Barsoumian A, et al. In vitro toxicity and activity of Dakin's solution, mafenide acetate, and amphotericin B on filamentous fungi and human cells. J Orthop Trauma. 2013 Aug;27(8):428-36. [Content Brief]

[4]. Baver Acaban M, et al. The effects of topical mafenide acetate application on skin graft survival in bacterial contaminated wounds. Burns. 2024 Mar;50(2):433-443. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.0604 mL	20.3021 mL	40.6042 mL	101.5105 mL
	5 mM	0.8121 mL	4.0604 mL	8.1208 mL	20.3021 mL
	10 mM	0.4060 mL	2.0302 mL	4.0604 mL	10.1510 mL
	15 mM	0.2707 mL	1.3535 mL	2.7069 mL	6.7674 mL
	20 mM	0.2030 mL	1.0151 mL	2.0302 mL	5.0755 mL
	25 mM	0.1624 mL	0.8121 mL	1.6242 mL	4.0604 mL
	30 mM	0.1353 mL	0.6767 mL	1.3535 mL	3.3837 mL
	40 mM	0.1015 mL	0.5076 mL	1.0151 mL	2.5378 mL
	50 mM	0.0812 mL	0.4060 mL	0.8121 mL	2.0302 mL
	60 mM	0.0677 mL	0.3384 mL	0.6767 mL	1.6918 mL
	80 mM	0.0508 mL	0.2538 mL	0.5076 mL	1.2689 mL
	100 mM	0.0406 mL	0.2030 mL	0.4060 mL	1.0151 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.