2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. MAFP

MAFP  (Synonyms: Methyl Arachidonyl Fluorophosphonate)

Cat. No.: HY-103334 Purity: 98.6%
COA
SDS
Handling Instructions Technical Support

MAFP (Methyl Arachidonyl Fluorophosphonate) is an selective, active-site directed and irreversible inhibitor of cPLA2 and iPLA2. MAFP is also a potent irreversible inhibitor of anandamide amidase.

For research use only. We do not sell to patients.

MAFP Chemical Structure

MAFP Chemical Structure

CAS No. : 188404-10-6

Size Price Stock Quantity
Solvent
1 mg (26.99 mM * 100 μL in Methyl acetate) In-stock
Solvent
5 mg (26.99 mM * 500 μL in Methyl acetate) In-stock
Solvent
10 mg (26.99 mM * 1 mL in Methyl acetate) In-stock
Solvent
25 mg (26.99 mM * 2.5 mL in Methyl acetate) In-stock
Solvent
50 mg (26.99 mM * 5 mL in Methyl acetate) Get quote
Solvent
100 mg (26.99 mM * 10 mL in Methyl acetate) Get quote

* Please select Quantity before adding items.

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Customer Review

Based on 5 publication(s) in Google Scholar

MAFP Related Antibodies

Top Publications Citing Use of Products

View All Phospholipase Isoform Specific Products:

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Phospholipase A PLD Phospholipase C

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

MAFP (Methyl Arachidonyl Fluorophosphonate) is an selective, active-site directed and irreversible inhibitor of cPLA2 and iPLA2. MAFP is also a potent irreversible inhibitor of anandamide amidase.

IC50 & Target

cPLA2, iPLA2[1], Anandamide amidase[2]
In Vitro

MAFP inhibits iPLA2, in a concentration-dependent manner with an IC50 of 0.5 μM after a 5 min preincubation at 40°C in P388D1 cells. cPLA, is a phospholipid hydrolase using the hydroxyl of serine-228 residue as its catalytic nucleophile[1]. MAFP is also an inhibitor of anandamide amidase and as a ligand for the CB1 cannabinoid receptor. MAFP demonstrates selectivity towards anandamide amidase for which it is approximately 3000 and 30000-fold more potent than it is towards chymotrypsin and trypsin, respectively. MAFP displaces [3H]CP-55940 binding to the CB1 cannabinoid receptor with an IC50 of 20 nM vs 40 nM for anandamide[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MAFP Related Antibodies
Molecular Weight

370.48

Formula

C21H36FO2P
CAS No.
Appearance

Liquid (Density: 0.960±0.06 g/cm3)

Color

Colorless to light yellow

SMILES

CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCCP(OC)(F)=O
Shipping

Shipping with dry ice.
Storage

-80°C, protect from light
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (269.92 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6992 mL 13.4960 mL 26.9920 mL
5 mM 0.5398 mL 2.6992 mL 5.3984 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light). When stored at -80°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (13.50 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (13.50 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.6%

SDS (604 KB)

COA (237 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[1]

MAFP is dissolved and diluted in DMSO. To investigate the reversibility of iPLA 2 inhibition by MAFP, the P388D1 iPLA 2 is first concentrated approximately 10-fold using a Centricon-10 concentrator from Amicon. The concentrated iPLA 2 (20 μL) is then preincubated with either 80 μM MAFP in DMSO or DMSO alone (2 μL) for 5 min at 40°C. A 2 μL aliquot is removed and subsequently diluted 1500-fold into 3 mL of assay mixture containing 100 μM DPPC (200000 cpm per 50 μL assay mixture), 400 μM Triton X-100, 100 mM Hepes (pH 7.5), 5 mM EDTA, 1 mM DTT and 0.8 mM ATP. At the indicated time points, a 50 μL aliquot is removed and the remaining enzyme activity is quantified[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay
[2]

Inhibition of anandamide amidase in cell culture is measured using approximately 1x106 Nl8TG2 intact neuroblastoma cells. Experimental cells are preincubated for 20 min in 1.5 mL medium, consisting of Fl2/DMEM with penicillin, streptomycin, gentamicin, 10% bovine calf serum, plus MAFP (1, 5, 10, 20 nM). Control cells contained no inhibitor. Arachidonoyl is then added and the incubation continued for I hr. The amount of [3H]anandamide in the cells is quantified by liquid scintillation counting of the silica scraped from the appropriate areas of the TLC plate identified by exposure to X-ray film[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light). When stored at -80°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6992 mL 13.4960 mL 26.9920 mL 67.4800 mL
5 mM 0.5398 mL 2.6992 mL 5.3984 mL 13.4960 mL
10 mM 0.2699 mL 1.3496 mL 2.6992 mL 6.7480 mL
15 mM 0.1799 mL 0.8997 mL 1.7995 mL 4.4987 mL
20 mM 0.1350 mL 0.6748 mL 1.3496 mL 3.3740 mL
25 mM 0.1080 mL 0.5398 mL 1.0797 mL 2.6992 mL
30 mM 0.0900 mL 0.4499 mL 0.8997 mL 2.2493 mL
40 mM 0.0675 mL 0.3374 mL 0.6748 mL 1.6870 mL
50 mM 0.0540 mL 0.2699 mL 0.5398 mL 1.3496 mL
60 mM 0.0450 mL 0.2249 mL 0.4499 mL 1.1247 mL
80 mM 0.0337 mL 0.1687 mL 0.3374 mL 0.8435 mL
100 mM 0.0270 mL 0.1350 mL 0.2699 mL 0.6748 mL
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MAFP Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MAFP188404-10-6Methyl Arachidonyl FluorophosphonatePhospholipaseInhibitorinhibitorinhibit

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