L-NMMA acetate (Synonyms: Tilarginine acetate; Methylarginine acetate)

Name: L-NMMA acetate
Brand: MedChemExpress
SKU: HY-18732A
Rating: 5.0 (8 reviews)

Cat. No.: HY-18732A Purity: ≥99.0%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

L-NMMA acetate is a nitric oxide synthase inhibitor of all NOS isoforms including NOS1, NOS2, and NOS3. The K_i values for nNOS (rat), eNOS (human), and iNOS (mouse) are approximately 0.18, 0.4, and 6 µM, respectively.

For research use only. We do not sell to patients.

L-NMMA acetate Chemical Structure

CAS No. : 53308-83-1

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
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100 mg	Get quote

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Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of L-NMMA acetate:

L-NMMA Get quote
L-NMMA citrate Get quote
L-NMMA hydrochloride Get quote

8 Publications Citing Use of MCE L-NMMA acetate

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View All NO Synthase Isoform Specific Products:

View All Isoforms

nNOS iNOS eNOS

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

eNOS

iNOS

nNOS

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	17 μM Compound: L-NMMA	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pre-incubated for 2 hrs before LPS stimulation for 24 hrs by nitrate/nitrite assay Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pre-incubated for 2 hrs before LPS stimulation for 24 hrs by nitrate/nitrite assay	[PMID: 31095383]
RAW264.7	IC₅₀	20.34 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by Griess reagent based assay Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by Griess reagent based assay	[PMID: 35503996]
RAW264.7	CC₅₀	214.41 μM Compound: L-NMMA	Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability measured after 12 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability measured after 12 hrs by MTT assay	[PMID: 35503996]
RAW264.7	IC₅₀	39.9 μM Compound: L-NMMA	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs by Griess assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs by Griess assay	[PMID: 28654256]
RAW264.7	IC₅₀	47.2 μM Compound: L-NMMA	Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 18 hrs by Griess reagent based assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 18 hrs by Griess reagent based assay	[PMID: 33325237]
RAW264.7	IC₅₀	78.3 μM Compound: L-NMMA	Inhibition of NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based analysis Inhibition of NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based analysis	[PMID: 34890996]

In Vitro

L-NMMA, starting from 100 μM, produces a concentration-dependent inhibition of the evoked relaxations (2Hz); maximal inhibition at 1 mM averaged about 35%. The inhibitory effect of L-NMMA is unchanged by previous incubation with D-arginine while it is prevented by L-arginine (L-Arg). L-NMMA does not affect isoprenaline-induced relaxation^[2]. Superfusion of L-NMMA reduces arteriolar diameter and causes dose-dependent increases in arteriolar tone. The onset of action of L-NMMA is nearly immediate. L-NMMA inhibits vasodilator responses to the endothelium-dependent vasodilator ACh but not to the endothelium-independent NP. NE induced dose-related vasoconstriction that is significantly potentiated by L-NMMA^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

248.28

Formula

C₉H₂₀N₄O₄

CAS No.

53308-83-1

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCCNC(NC)=N)C(O)=O.O=C(C)O

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 50 mg/mL (201.39 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0277 mL	20.1386 mL	40.2771 mL
5 mM	0.8055 mL	4.0277 mL	8.0554 mL
10 mM	0.4028 mL	2.0139 mL	4.0277 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (402.77 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (246 KB)

Handling Instructions (2659 KB)

References

[1]. Frey C, et al. L-thiocitrulline. A stereospecific, heme-binding inhibitor of nitric-oxide synthases. J Biol Chem. 1994 Oct 21;269(42):26083-91. [Content Brief]

[2]. Maggi CA, et al. Effect of NG-monomethyl L-arginine (L-NMMA) and NG-nitro L-arginine (L-NOARG) on non-adrenergic non-cholinergic relaxation in the circular muscle of the human ileum. Br J Pharmacol. 1991 Aug;103(4):1970-2. [Content Brief]

[3]. Nakamura T, et al. Effect of NG-monomethyl-L-arginine on arcade arterioles of rat spinotrapezius muscles. Am J Physiol. 1991 Jul;261(1 Pt 2):H46-52. [Content Brief]

Kinase Assay
^[1]

NOS activity is determined by monitoring the conversion of L-[¹⁴C]arginine to [¹⁴C]citrulline. For nNOS, reaction mixtures contain, in a final volume of 200 μL, 20 mM Na⁺ HEPES buffer, pH 7.5, 100 μM EDTA, 2 mM CaCl₂, 10 μg/mL calmodulin, 500 μM dithiothreitol, 100 μM THB, 25 μM FAD, 25 μM FMN, 100 μg/mL bovine serum albumin, 20 μM L-[¹⁴C]citrulline, 500 PM NADPH, and enzyme. Reaction is initiated by the addition of L-[¹⁴C]citrulline; reaction temperature is 25°C. At appropriate times, typically 3.5, 7, and 10.5 min, 50-1.11 portions are removed and added to 200 pl of a stopping solution of 100 mM Na⁺ HEPES buffer, pH 5.5, containing 5 mM EGTA. Those samples are immediately heated for 1 min in a boiling water bath, chilled, and centrifuged. A portion (225 μL) of the supernatant is fractionated on small Dowex 50 columns. [¹⁴C]citrulline is eluted with 2 ml of water and is quantitated by liquid scintillation counting^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Frey C, et al. L-thiocitrulline. A stereospecific, heme-binding inhibitor of nitric-oxide synthases. J Biol Chem. 1994 Oct 21;269(42):26083-91. [Content Brief]

[2]. Maggi CA, et al. Effect of NG-monomethyl L-arginine (L-NMMA) and NG-nitro L-arginine (L-NOARG) on non-adrenergic non-cholinergic relaxation in the circular muscle of the human ileum. Br J Pharmacol. 1991 Aug;103(4):1970-2. [Content Brief]

[3]. Nakamura T, et al. Effect of NG-monomethyl-L-arginine on arcade arterioles of rat spinotrapezius muscles. Am J Physiol. 1991 Jul;261(1 Pt 2):H46-52. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.0277 mL	20.1386 mL	40.2771 mL	100.6928 mL
	5 mM	0.8055 mL	4.0277 mL	8.0554 mL	20.1386 mL
	10 mM	0.4028 mL	2.0139 mL	4.0277 mL	10.0693 mL
	15 mM	0.2685 mL	1.3426 mL	2.6851 mL	6.7129 mL
	20 mM	0.2014 mL	1.0069 mL	2.0139 mL	5.0346 mL
	25 mM	0.1611 mL	0.8055 mL	1.6111 mL	4.0277 mL
	30 mM	0.1343 mL	0.6713 mL	1.3426 mL	3.3564 mL
	40 mM	0.1007 mL	0.5035 mL	1.0069 mL	2.5173 mL
	50 mM	0.0806 mL	0.4028 mL	0.8055 mL	2.0139 mL
	60 mM	0.0671 mL	0.3356 mL	0.6713 mL	1.6782 mL
	80 mM	0.0503 mL	0.2517 mL	0.5035 mL	1.2587 mL
	100 mM	0.0403 mL	0.2014 mL	0.4028 mL	1.0069 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

L-NMMA acetate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-NMMA53308-83-1TilarginineMethylarginineNO SynthaseEndogenous MetaboliteNitric oxide synthasesNOSInhibitorinhibitorinhibit

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L-NMMA acetate (Synonyms: Tilarginine acetate; Methylarginine acetate)

Customer Review

Other Forms of L-NMMA acetate:

L-NMMA acetate Related Antibodies

8 Publications Citing Use of MCE L-NMMA acetate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NO Synthase Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

L-NMMA acetate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

L-NMMA acetate Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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