2. Anti-infection MAPK/ERK Pathway Apoptosis Autophagy
  3. Fungal JNK p38 MAPK Bcl-2 Family Caspase Autophagy Apoptosis
  4. Fluazinam

Fluazinam is an orally active dinitroaniline fungicide. Fluazinam induces phosphorylation of JNK, activates p38 pathway, decreases Bcl-2, activates caspase-3, decreases complex I activity, increases Autophagy and Apoptosis. Fluazinam has strong antifungal activity against F. fujikuroi and B. maydis. Fluazinam has a negative impact on Brachydanio rerio and worker bees.

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Fluazinam Chemical Structure

Fluazinam Chemical Structure

CAS No. : 79622-59-6

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Based on 1 publication(s) in Google Scholar

Other Forms of Fluazinam:

Fluazinam Related Antibodies

Top Publications Citing Use of Products

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JNK1 JNK2 JNK3 JNK

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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Description

Fluazinam is an orally active dinitroaniline fungicide. Fluazinam induces phosphorylation of JNK, activates p38 pathway, decreases Bcl-2, activates caspase-3, decreases complex I activity, increases Autophagy and Apoptosis. Fluazinam has strong antifungal activity against F. fujikuroi and B. maydis. Fluazinam has a negative impact on Brachydanio rerio and worker bees[1][2][3][4][5][6][7].

IC50 & Target

Fungal[1]
In Vitro

Fluazinam (0-5 μM; 24 h) induces SH-SY5Y cell death and nuclear damage[1].
Fluazinam (10-20 μg/mL) has great control efficiency against B. maydis on detached corn leaves[2].
Fluazinam has strong antifungal activity against F. fujikuroi, with EC50 values ranging from 0.0621 to 0.5446 μg/mL for mycelial growth inhibition of 103 F. fujikuroi isolates[3].
Fluazinam (0.01-0.5 μM) impairs oxidative phosphorylation and induces hyper/hypo-activity in a dose specific manner in zebrafish larvae[4].
Fluazinam (0.03-0.40 mg/L; 24-96 h) has acute toxicity to Brachydanio rerio[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fluazinam Related Antibodies

Western Blot Analysis[1]

Cell Line: SH-SY5Y
Concentration: 1.25, 2.5, 5 μM
Incubation Time: 10 min, 30 min, 1 h, 2 h, 4 h, 8 h, 16 h, 24 h
Result: Induced phosphorylation of JNK.
Activated the p38 pathway.
Induced a decrease in the level of Bcl-2 protein.
Activated caspase-3 in a dose-dependent manner.
Decreased complex I activity.
In Vivo

Fluazinam (0.5-2.5 mg a.i. mL-1; oral exposure; 24 h) reduces food consumption, damages the midgut epithelium, increases autophagy and apoptosis in the midgut, induces oxidative stress, and alters the walking behavior in Partamona helleri worker bees[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

465.09

Formula

C13H4Cl2F6N4O4
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

FC(C1=CN=C(NC2=C([N+]([O-])=O)C=C(C(F)(F)F)C(Cl)=C2[N+]([O-])=O)C(Cl)=C1)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (268.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1501 mL 10.7506 mL 21.5012 mL
5 mM 0.4300 mL 2.1501 mL 4.3002 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (4.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1501 mL 10.7506 mL 21.5012 mL 53.7530 mL
5 mM 0.4300 mL 2.1501 mL 4.3002 mL 10.7506 mL
10 mM 0.2150 mL 1.0751 mL 2.1501 mL 5.3753 mL
15 mM 0.1433 mL 0.7167 mL 1.4334 mL 3.5835 mL
20 mM 0.1075 mL 0.5375 mL 1.0751 mL 2.6877 mL
25 mM 0.0860 mL 0.4300 mL 0.8600 mL 2.1501 mL
30 mM 0.0717 mL 0.3584 mL 0.7167 mL 1.7918 mL
40 mM 0.0538 mL 0.2688 mL 0.5375 mL 1.3438 mL
50 mM 0.0430 mL 0.2150 mL 0.4300 mL 1.0751 mL
60 mM 0.0358 mL 0.1792 mL 0.3584 mL 0.8959 mL
80 mM 0.0269 mL 0.1344 mL 0.2688 mL 0.6719 mL
100 mM 0.0215 mL 0.1075 mL 0.2150 mL 0.5375 mL
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Fluazinam Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fluazinam79622-59-6FungalJNKp38 MAPKBcl-2 FamilyCaspaseAutophagyApoptosisc-Jun N-terminal kinaseworker beeszebrafishF. fujikuroiantifungalInhibitorinhibitorinhibit

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