Dihydromethysticin (Synonyms: (+)-Dihydromethysticin)

Name: Dihydromethysticin
Brand: MedChemExpress
SKU: HY-N0921
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0921 Purity: 99.94%: Data Sheet
COA

SDS
Handling Instructions
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Dihydromethysticin is an orally active natural active ingredient. Dihydromethysticin can be extracted from Piper methysticum. Dihydromethysticin inhibits carboxylesterase 1 (K_i = 68.2 μM) and CYP2A5. Dihydromethysticin upregulates NLRC3 and induces Apoptosis. Dihydromethysticin exhibits anticancer activity against colorectal cancer and lung adenoma.

For research use only. We do not sell to patients.

Dihydromethysticin Chemical Structure

CAS No. : 19902-91-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Dihydromethysticin:

Dihydromethysticin (Standard) Get quote

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View All Carboxylesterase (CES) Isoform Specific Products:

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CES1 CES2 CES3

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NLRP3 NLRP1 NOD1 NOD2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]^[3]^[4]

CES1

68.2 μM (Ki)

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	20 μg/mL Compound: (+)-Dihydromethysticin	Inhibition of TNF-alpha-induced NF-kappaB expressed in human A549 cells treated 1 hr after TNFalpha challenge measured after 6 hrs by luciferase reporter gene assay Inhibition of TNF-alpha-induced NF-kappaB expressed in human A549 cells treated 1 hr after TNFalpha challenge measured after 6 hrs by luciferase reporter gene assay	[PMID: 19716299]

In Vitro

Dihydromethysticin (0-400 μM; 24-48 h) inhibits proliferation, migration, invasion, promotes apoptosis and G0/G1 arrest in colorectal cancer cells via the NLRC3/PI3K pathway^[1].
Dihydromethysticin (0-208.46 μM) inhibits carboxylesterase 1 with mixed competitive-noncompetitive type inhibition (K_i = 68.2 μM)^[2].
Dihydromethysticin (10-100 μM) inhibits coumarin 7-hydroxylation (CYP2A5) activity in mouse liver microsomes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HCT116, HT29, LoVo (colorectal cancer cells); NCM460 (normal colonic mucosal cells)
Concentration:	0, 25, 50, 100, 200, 400 μM
Incubation Time:	24, 48 h
Result:	Suppressed proliferation in all CRC cells in a dose- and time- dependent manner. Did not show inhibitory effect on NCM460 cells.

In Vivo

Dihydromethysticin (10 mg/kg; i.p.; daily for 4 weeks) suppresses tumor growth and angiogenesis in BALB/c nude mice with subcutaneous CRC via NLRC3/PI3K pathway, without toxicity^[1].
Dihydromethysticin (0.8 mg/mouse; p.o.; before NNK injection) completely inhibits lung adenoma formation in A/J mice by reducing DNA adducts and enhancing NNAL detoxification^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male BALB/c nude mice (6-week-old) with subcutaneous CRC (HCT116/HT29 cells)^[1]
Dosage:	10 mg/kg
Administration:	Intraperitoneal injection, daily for 4 weeks
Result:	Reduced tumor volume. Decreased Ki-67/CD31 expression (proliferation/angiogenesis). Increased TUNEL-positive cells (apoptosis). Upregulated NLRC3 and downregulated p-PI3K/p-mTOR in tumors.

Molecular Weight

276.29

Formula

C₁₅H₁₆O₅

CAS No.

19902-91-1

Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(OC)C[C@H](CCC2=CC=C(OCO3)C3=C2)O1

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (361.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6194 mL	18.0971 mL	36.1941 mL
5 mM	0.7239 mL	3.6194 mL	7.2388 mL
10 mM	0.3619 mL	1.8097 mL	3.6194 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (284 KB) SDS (252 KB)

COA (247 KB) HNMR (252 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Pan H, et al. Dihydromethysticin, a natural molecule from Kava, suppresses the growth of colorectal cancer via the NLRC3/PI3K pathway. Mol Carcinog. 2020 Jun;59(6):575-589. [Content Brief]

[2]. Melchert PW, et al. In vitro inhibition of carboxylesterase 1 by Kava (Piper methysticum) Kavalactones. Chem Biol Interact. 2022 Apr 25;357:109883. [Content Brief]

[3]. Narayanapillai SC, et al. Dietary Dihydromethysticin Increases Glucuronidation of 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanol in A/J Mice, Potentially Enhancing Its Detoxification. Drug Metab Dispos. 2016 Mar;44(3):422-7. [Content Brief]

[4]. Hu Q, et al. Oral Dosing of Dihydromethysticin Ahead of Tobacco Carcinogen NNK Effectively Prevents Lung Tumorigenesis in A/J Mice. Chem Res Toxicol. 2020 Jul 20;33(7):1980-1988. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6194 mL	18.0971 mL	36.1941 mL	90.4853 mL
	5 mM	0.7239 mL	3.6194 mL	7.2388 mL	18.0971 mL
	10 mM	0.3619 mL	1.8097 mL	3.6194 mL	9.0485 mL
	15 mM	0.2413 mL	1.2065 mL	2.4129 mL	6.0324 mL
	20 mM	0.1810 mL	0.9049 mL	1.8097 mL	4.5243 mL
	25 mM	0.1448 mL	0.7239 mL	1.4478 mL	3.6194 mL
	30 mM	0.1206 mL	0.6032 mL	1.2065 mL	3.0162 mL
	40 mM	0.0905 mL	0.4524 mL	0.9049 mL	2.2621 mL
	50 mM	0.0724 mL	0.3619 mL	0.7239 mL	1.8097 mL
	60 mM	0.0603 mL	0.3016 mL	0.6032 mL	1.5081 mL
	80 mM	0.0452 mL	0.2262 mL	0.4524 mL	1.1311 mL
	100 mM	0.0362 mL	0.1810 mL	0.3619 mL	0.9049 mL

Dihydromethysticin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dihydromethysticin19902-91-1(+)-DihydromethysticinCarboxylesterase (CES)NOD-like Receptor (NLR)ApoptosisCarboxylesteraseCarboxylic-ester Hydrolaselung tumorigenesismiceCES1HCT116HT29and LoVoInhibitorinhibitorinhibit

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Dihydromethysticin (Synonyms: (+)-Dihydromethysticin)

Customer Review

Other Forms of Dihydromethysticin:

Dihydromethysticin Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Carboxylesterase (CES) Isoform Specific Products:

View All NOD-like Receptor (NLR) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Dihydromethysticin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Dihydromethysticin Related Classifications

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