2. Metabolic Enzyme/Protease Apoptosis Immunology/Inflammation Anti-infection
  3. Lipoxygenase TNF Receptor Interleukin Related COX Fungal Parasite Apoptosis
  4. 7,3',4'-Tri-O-methylluteolin

7,3',4'-Tri-O-methylluteolin  (Synonyms: 5-Hydroxy-3',4',7-trimethoxyflavone)

Cat. No.: HY-N7012 Purity: 98.95%
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COA
Handling Instructions Technical Support

7,3',4'-Tri-O-methylluteolin (5-Hydroxy-3',4',7-trimethoxyflavone) is a flavonoid with multiple biological activities. 7,3',4'-Tri-O-methylluteolin inhibits soybean lipoxygenase (LOX), with an IC50 value of 23.97 µg/mL. 7,3',4'-Tri-O-methylluteolin possesses anti-inflammatory effects in Lipopolysaccharides (HY-D1056) (LPS)-induced RAW 264.7 macrophages. 7,3',4'-Tri-O-methylluteolin inhibits the binding of MDM2 with p53 and induces apoptosis in MCF-7 breast cancer cells. 7,3',4'-Tri-O-methylluteolin also has antioxidant, antifungal and antitrypanosomal activitiessup>[4]sup>[5].

For research use only. We do not sell to patients.

7,3',4'-Tri-O-methylluteolin Chemical Structure

7,3',4'-Tri-O-methylluteolin Chemical Structure

CAS No. : 29080-58-8

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Based on 1 publication(s) in Google Scholar

7,3',4'-Tri-O-methylluteolin Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

7,3',4'-Tri-O-methylluteolin (5-Hydroxy-3',4',7-trimethoxyflavone) is a flavonoid with multiple biological activities. 7,3',4'-Tri-O-methylluteolin inhibits soybean lipoxygenase (LOX), with an IC50 value of 23.97 µg/mL. 7,3',4'-Tri-O-methylluteolin possesses anti-inflammatory effects in Lipopolysaccharides (HY-D1056) (LPS)-induced RAW 264.7 macrophages. 7,3',4'-Tri-O-methylluteolin inhibits the binding of MDM2 with p53 and induces apoptosis in MCF-7 breast cancer cells. 7,3',4'-Tri-O-methylluteolin also has antioxidant, antifungal and antitrypanosomal activities[1][2][3]sup>[4]sup>[5].

IC50 & Target[1]

IL-6

 

IL-1β

 

COX-2

 

Cellular Effect
Cell Line Type Value Description References
HepG2 IC50
15 μM
Compound: 6
Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
[PMID: 23800391]
MCF7 IC50
10 μM
Compound: 6
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
[PMID: 23800391]
Vero IC50
125 μM
Compound: 6
Antiproliferative activity against african green monkey Vero cells after 48 hrs by MTT assay
Antiproliferative activity against african green monkey Vero cells after 48 hrs by MTT assay
[PMID: 23800391]
WI-38 IC50
105 μM
Compound: 6
Antiproliferative activity against human WI38 cells after 48 hrs by MTT assay
Antiproliferative activity against human WI38 cells after 48 hrs by MTT assay
[PMID: 23800391]
In Vitro

In LPS (1 µg/mL)-induced RAW 264.7 macrophages, 7,3',4'-Tri-O-methylluteolin (5-Hydroxy-3',4',7-trimethoxyflavone) (1-40 µg/mL) significantly inhibits nitric oxide production and demonstrats slight reduction in prostaglandin-E2 level at tested concentrations. 7,3',4'-Tri-O-methylluteolin reduces the production of pro-inflammatory cytokines, such as TNF-α, IL-6, and IL-1β[1].
7,3',4'-Tri-O-methylluteolin (40 µg/mL; 24 h) significantly induces reduction in the mRNA expressions of iNOS synthase and COX-2 in RAW 264.7 cells[1].
7,3',4'-Tri-O-methylluteolin has antioxidant effects and can scavenge free radicals, superoxides, and hydroxyl radicals[2].
7,3',4'-Tri-O-methylluteolin (5-15 μg/mL; 24-48 h) induces cytotoxicity in the treated cells, which correspondingly resulted in the IC50 values of 12 μg/mL and 8 μg/mL after 24 and 48 h treatment[3].
7,3',4'-Tri-O-methylluteolin (8-12 μg/mL; 24-48 h) induces apoptosis in MCF-7 cells. 7,3',4'-Tri-O-methylluteolin induces the phosphatidylserine translocation, DNA damage and the significant elevation of cellular ROS[3].
7,3',4'-Tri-O-methylluteolin (8-12 μg/mL; 24-48 h) encourages apoptosis through the modulation of apoptotic markers, such as p53, Bcl-2, Bax, and cleaved PARP. 7,3',4'-Tri-O-methylluteolin shifts the Bax:Bcl-2 ratio[3].
7,3',4'-Tri-O-methylluteolin shows activities against Candida albicans, Candida krusei and Candida galabrata[4].
7,3',4'-Tri-O-methylluteolin inhibits Trypanosoma brucei with a MIC value of 19.0 μM[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7,3',4'-Tri-O-methylluteolin Related Antibodies

Cell Viability Assay[3]

Cell Line: MCF-7 cells
Concentration: 5 μg/mL, 6 μg/mL, 7 μg/mL, 8 μg/mL, 9 μg/mL, 10 μg/mL, 11 μg/mL, 12 μg/mL, 13 μg/mL, 14 μg/mL, 15 μg/mL
Incubation Time: 24 and 48 h
Result: Showed anti-proliferative effects in MCF-7 cells.

Apoptosis Analysis[3]

Cell Line: MCF-7 cells
Concentration: 8 μg/mL,12 μg/mL
Incubation Time: 24 and 48 h
Result: Clearly showed typical apoptotic features, such as cell shrinkage, membrane blebbing, distorted nuclei, chromatin fragmentation, and formation of pyknotic bodies of condensed chromatin, comparatively with a bright green/yellow and orange/red staining.

Western Blot Analysis[3]

Cell Line: MCF-7 cells
Concentration: 8 μg/mL,12 μg/mL
Incubation Time: 24 and 48 h
Result: The up-regulation of pro-apoptotic proteins Bax and PARP cleavage and down-regulation of anti-apoptotic protein Bcl-2.

RT-PCR[3]

Cell Line: MCF-7 cells
Concentration: 8 μg/mL,12 μg/mL
Incubation Time: 24 and 48 h
Result: Showed a several-fold increase in the transcripts of p53, p21, and Bax in a time-dependent manner.

RT-PCR[1]

Cell Line: RAW 264.7 cells treated with LPS (1 µg/mL)
Concentration: 40 µg/mL
Incubation Time: 24 h
Result: Significantly induced reduction in the mRNA expressions of inducible nitric oxide (iNOS) synthase and COX-2.
Molecular Weight

328.32

Formula

C18H16O6
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

OC1=C2C(OC(C3=CC=C(OC)C(OC)=C3)=CC2=O)=CC(OC)=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (60.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0458 mL 15.2290 mL 30.4581 mL
5 mM 0.6092 mL 3.0458 mL 6.0916 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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C2

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Volume (final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2 mg/mL (6.09 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.28%

SDS (252 KB)

COA (248 KB) HNMR (103 KB) RP-HPLC (103 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0458 mL 15.2290 mL 30.4581 mL 76.1452 mL
5 mM 0.6092 mL 3.0458 mL 6.0916 mL 15.2290 mL
10 mM 0.3046 mL 1.5229 mL 3.0458 mL 7.6145 mL
15 mM 0.2031 mL 1.0153 mL 2.0305 mL 5.0763 mL
20 mM 0.1523 mL 0.7615 mL 1.5229 mL 3.8073 mL
25 mM 0.1218 mL 0.6092 mL 1.2183 mL 3.0458 mL
30 mM 0.1015 mL 0.5076 mL 1.0153 mL 2.5382 mL
40 mM 0.0761 mL 0.3807 mL 0.7615 mL 1.9036 mL
50 mM 0.0609 mL 0.3046 mL 0.6092 mL 1.5229 mL
60 mM 0.0508 mL 0.2538 mL 0.5076 mL 1.2691 mL
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7,3',4'-Tri-O-methylluteolin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7,3',4'-Tri-O-methylluteolin29080-58-85-Hydroxy-3',4',7-trimethoxyflavoneLipoxygenaseTNF ReceptorInterleukin RelatedCOXFungalParasiteApoptosisLOXTumor Necrosis Factor ReceptorTNFRILCyclooxygenaseInflammation-related diseaseslipopolysaccharideRAW 264.7 macrophagepro-inflammatory cytokinesInhibitorinhibitorinhibit

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