5-FITC tyramide 

5-FITC tyramide is a green fluorescent dye composed of FITC (HY-66019) and tyramide (Ex=488 nm, Em=525 nm). 5-FITC tyramide is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). 5-FITC tyramide can be used for multiplex immunohistochemistry (mIHC)^[1].

5-FITC tyramide assay
a. Inject into the rat ganglion with 4 μL of WGA-HRP tracer, and then get specific tissues for slide.
b. Slides were allowed to dry for 60 min.
c. Rehydrate in TNT buffer (0.1 M Tris-HCl, 0.15 M NaCl, 0.05% Tween 20, pH 7.5), and then quench in freshly prepared NaBH₄ (0.5 mg/mL) in TN buffer (0.1 M Tris-HCl, 0.15 M NaCl, pH 7.5) for 15 min.
d. Wash in TNT and then permeabilized in 0.1% Triton X-100 in TN for 15 min.
e. Incubate in TNT plus 2% BSA (TNT-BSA) for 60 min following washing in TNT, and then incubate min in 5-FITC tyramide for 15 min (1: 50 with amplification diluent).
f. Wash in TNT, and then apply the primary antibodies overnight at 4℃ following detecting by incubation with donkey anti-rabbit IgG conjugated to Lissamine rhodamine (LRSC) or Cy3 (HY-D0822) (1:200 with TNT-BSA) and donkey anti-sheep Cy5 (HY-D0821) (1:200 with TNT-BSA).
g. Fluorescence microscopy detection.
Note: it is suggested that the optimal concentration of 5-FITC tyramide working solution according to actual conditions.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

526.56

C₂₉H₂₂N₂O₆S

OC1=CC=C(CCNC(NC2=CC=C(C3(OC4=O)C5=CC=C(C=C5OC6=CC(O)=CC=C63)O)C4=C2)=S)C=C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kressel M. Tyramide amplification allows anterograde tracing by horseradish peroxidase-conjugated lectins in conjunction with simultaneous immunohistochemistry. J Histochem Cytochem. 1998 Apr; 46(4):527-33.  [Content Brief]