2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. COX Drug Metabolite
  4. Ampyrone

Ampyrone  (Synonyms: 4-Aminophenazone)

Cat. No.: HY-B1398 Purity: 99.72%
SDS
COA
Handling Instructions Technical Support

Ampyrone (4-Aminophenazone; 4-Aminoantipyrine) is a reversible and low-damage optical clearing agent and non-selective COX inhibitor based on UV absorption properties. Ampyrone can improve the optical transmittance of mouse skin and other tissues. Ampyrone can induce tissue refractive index matching by enhancing UV absorption, reduce light scattering, and achieve tissue transparency in vivo. Ampyrone reduces the synthesis of prostaglandin PGE2, thereby exerting anti-inflammatory, analgesic and antipyretic effects. Ampyrone inhibits DNA damage, cell apoptosis and immune cell phagocytosis induced by Doxorubicin (HY-15142A) and Cisplatin (HY-17394), etc., and participates in the regulation of toxicity in tumor chemotherapy.

For research use only. We do not sell to patients.

Ampyrone Chemical Structure

Ampyrone Chemical Structure

CAS No. : 83-07-8

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
5 g   Get quote  
10 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Ampyrone:

Ampyrone Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Ampyrone

View All COX Isoform Specific Products:

View All Isoforms
COX COX-1 COX-2 COX-3

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ampyrone (4-Aminophenazone; 4-Aminoantipyrine) is a reversible and low-damage optical clearing agent and non-selective COX inhibitor based on UV absorption properties. Ampyrone can improve the optical transmittance of mouse skin and other tissues. Ampyrone can induce tissue refractive index matching by enhancing UV absorption, reduce light scattering, and achieve tissue transparency in vivo. Ampyrone reduces the synthesis of prostaglandin PGE2, thereby exerting anti-inflammatory, analgesic and antipyretic effects. Ampyrone inhibits DNA damage, cell apoptosis and immune cell phagocytosis induced by Doxorubicin (HY-15142A) and Cisplatin (HY-17394), etc., and participates in the regulation of toxicity in tumor chemotherapy[1][2][3].

IC50 & Target[1]

COX

 

In Vivo

Ampyrone (12-24 mg/kg; intraperitoneal injection; single dose; 72 h) is non-genotoxic and non-mutagenic in male adult mice. However, when Ampyrone is used in combination with Doxorubicin (HY-15142A), Cisplatin (HY-17394) or Cyclophosphamide (HY-17420), Ampyrone reduces the induced DNA damage, micronucleus formation, cell apoptosis and spleen phagocytic activity[1].
Ampyrone supports structural (such as YFP-labeled neurons) and functional (such as GCaMP calcium signals) imaging of deep tissues (such as the cerebral cortex), and is suitable for longitudinal dynamic observations in living animals, such as long-term tracking of neurons in the awake mouse brain and real-time monitoring of neural activity[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Mus musculus (35 g, 8-10 weeks old)[1]
Dosage: 12-24 mg/kg
Administration: Intraperitoneal injection, single dose, 24 h
Result: Showed no genotoxicity, while co-administration with doxorubicin (24 mg/kg) or cyclophosphamide reduced DNA damage by 29.71%-62.19%.
Slight increase in micronuclei frequency was observed at 72 h with 24 mg/kg alone, but co-administration with cisplatin or doxorubicin reduced micronuclei formation by 3.22%-66.06%.
Reduced cisplatin/cyclophosphamide-induced apoptotic cells in kidneys (23.63%-47.11%) and doxorubicin-induced apoptosis in liver (18.18%-68.84%).
Co-administration with chemotherapeutic drugs decreased phagocytic cell numbers by 2.11%-63.76%, indicating immunomodulatory effects.
Molecular Weight

203.25

Formula

C11H13N3O
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1N(C2=CC=CC=C2)N(C)C(C)=C1N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (246.00 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (246.00 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.9200 mL 24.6002 mL 49.2005 mL
5 mM 0.9840 mL 4.9200 mL 9.8401 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 150 mg/mL (738.01 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.72%

SDS (392 KB)

COA (251 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 4.9200 mL 24.6002 mL 49.2005 mL 123.0012 mL
5 mM 0.9840 mL 4.9200 mL 9.8401 mL 24.6002 mL
10 mM 0.4920 mL 2.4600 mL 4.9200 mL 12.3001 mL
15 mM 0.3280 mL 1.6400 mL 3.2800 mL 8.2001 mL
20 mM 0.2460 mL 1.2300 mL 2.4600 mL 6.1501 mL
25 mM 0.1968 mL 0.9840 mL 1.9680 mL 4.9200 mL
30 mM 0.1640 mL 0.8200 mL 1.6400 mL 4.1000 mL
40 mM 0.1230 mL 0.6150 mL 1.2300 mL 3.0750 mL
50 mM 0.0984 mL 0.4920 mL 0.9840 mL 2.4600 mL
60 mM 0.0820 mL 0.4100 mL 0.8200 mL 2.0500 mL
80 mM 0.0615 mL 0.3075 mL 0.6150 mL 1.5375 mL
100 mM 0.0492 mL 0.2460 mL 0.4920 mL 1.2300 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Ampyrone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ampyrone83-07-84-AminophenazoneCOXDrug MetaboliteCyclooxygenaseInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Ampyrone
Cat. No.:
HY-B1398
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (392 KB) Français - FR (392 KB) Deutsch - DE (392 KB) Norwegian - NO (392 KB) Español - ES (392 KB) Swedish - SV (392 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.