2-(Aminomethyl)phenol (Synonyms: 2-Hydroxybenzylamine; o-Hydroxybenzylamine; 2-HOBA)

Name: 2-(Aminomethyl)phenol
Brand: MedChemExpress
SKU: HY-34350
Rating: 4.5 (1 reviews)

Cat. No.: HY-34350 Purity: 99.45%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

2-(Aminomethyl)phenol (2-Hydroxybenzylamine) is a selective dicarbonyl scavenger. 2-(Aminomethyl)phenol is an antioxidant and scavanger of free radicals and isolevuglandins (IsoLGs). 2-(Aminomethyl)phenol can prevent early recurrence of atrial fibrillation. 2-(Aminomethyl)phenol can reduce inflammation and plaque apoptotic cells and promote efferocytosis and features of stable plaques. 2-(Aminomethyl)phenol can reduce malondialdehyde (MDA)-LDL and MDA-HDL levels in Ldlr^-/- mouse model. 2-(Aminomethyl)phenol can be studied in the research of inflammation and cardiovascular disease, such as atherosclerosis, early recurrence of atrial fibrillation (AF) and arrhythmias.

For research use only. We do not sell to patients.

2-(Aminomethyl)phenol Chemical Structure

CAS No. : 932-30-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
> 100 g	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

2-(Aminomethyl)phenol (Compound 2-HOBA) (500 μM, 24 h) markedly decreases the number of apoptotic cells in both macrophage and human aortic endothelial cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[2]

Cell Line:	Peritoneal macrophages
Concentration:	500 μM
Incubation Time:	24 h
Result:	Significantly reduced the macrophage inflammatory response to oxidized LDL as shown by the decreased mRNA levels of IL-1β, IL-6, and TNF-α.

In Vivo

2-(Aminomethyl)phenol (Compound 2-HOBA) (1 g/L, p.o., 2 w pretreatment and 16 w afterwards) reduces the extent of proximal aortic atherosclerosis by approximately 31% in female Ldlr^-/- mouse model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Ldlr^-/- mouse model^[2]
Dosage:	1 g/L
Administration:	Oral gavage (p.o.) in diet, 2 w pretreatment and 16 w after
Result:	Reduced the extent of aortic atherosclerosis. Did not affect body weight, diet uptake and water consumption. Decreased the MDA and IsoLG adduct content by 59% and 23%. Increased the collagen content of the proximal aorta. Promoted efferocytosis and reduces inflammation.

Clinical Trial

Molecular Weight

123.16

Formula

C₇H₉NO

CAS No.

932-30-9

Appearance

Solid

Color

Light yellow to brown

SMILES

OC1=CC=CC=C1CN

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (405.98 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.1195 mL	40.5976 mL	81.1952 mL
5 mM	1.6239 mL	8.1195 mL	16.2390 mL
10 mM	0.8120 mL	4.0598 mL	8.1195 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (20.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (20.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.45%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) HNMR (224 KB) RP-HPLC (261 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Matthew J O'Neill, et al. 2-Hydroxybenzylamine (2-HOBA) to prevent early recurrence of atrial fibrillation after catheter ablation: protocol for a randomized controlled trial including detection of AF using a wearable device. [Content Brief]

[2]. Huan Tao, et al. Scavenging of reactive dicarbonyls with 2-hydroxybenzylamine reduces atherosclerosis in hypercholesterolemic Ldlr -/- mice. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	8.1195 mL	40.5976 mL	81.1952 mL	202.9880 mL
	5 mM	1.6239 mL	8.1195 mL	16.2390 mL	40.5976 mL
	10 mM	0.8120 mL	4.0598 mL	8.1195 mL	20.2988 mL
	15 mM	0.5413 mL	2.7065 mL	5.4130 mL	13.5325 mL
	20 mM	0.4060 mL	2.0299 mL	4.0598 mL	10.1494 mL
	25 mM	0.3248 mL	1.6239 mL	3.2478 mL	8.1195 mL
	30 mM	0.2707 mL	1.3533 mL	2.7065 mL	6.7663 mL
	40 mM	0.2030 mL	1.0149 mL	2.0299 mL	5.0747 mL
	50 mM	0.1624 mL	0.8120 mL	1.6239 mL	4.0598 mL
	60 mM	0.1353 mL	0.6766 mL	1.3533 mL	3.3831 mL
	80 mM	0.1015 mL	0.5075 mL	1.0149 mL	2.5373 mL
	100 mM	0.0812 mL	0.4060 mL	0.8120 mL	2.0299 mL

2-(Aminomethyl)phenol Related Classifications

Inflammation/Immunology Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-(Aminomethyl)phenol932-30-92-Hydroxybenzylamine o-Hydroxybenzylamine 2-HOBAReactive Oxygen Species (ROS)IsoLGsinflammationoxidative stressantioxidantscavengerradicalatrial fibrillationarrhythmiasatherosclerosisFH modelInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

2-(Aminomethyl)phenol (Synonyms: 2-Hydroxybenzylamine; o-Hydroxybenzylamine; 2-HOBA)

Customer Review

2-(Aminomethyl)phenol Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

2-(Aminomethyl)phenol Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

2-(Aminomethyl)phenol Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report