(Z-LL)2 ketone

Name: (Z-LL)2 ketone
Brand: MedChemExpress
SKU: HY-P1425
Rating: 4.5 (1 reviews)

Cat. No.: HY-P1425 Purity: 99.67%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

(Z-LL)2 ketone is an inhibitor of ketone with an IC₅₀ value of 50 nM. (Z-LL)2 ketone inhibits processing of thep-Prlsignal peptide.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

(Z-LL)2 ketone Chemical Structure

CAS No. : 313664-40-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

(Z-LL)2 ketone is an inhibitor of ketone with an IC₅₀ value of 50 nM. (Z-LL)2 ketone inhibits processing of thep-Prlsignal peptide^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	> 10 μM Compound: 41; (Z-LL)2	Inhibition of human gamma-secretase expressed in HEK293 cells using Notch1-VP16-Gal4 as substrate after 24 hrs by Bright-Glo luciferase reporter gene assay Inhibition of human gamma-secretase expressed in HEK293 cells using Notch1-VP16-Gal4 as substrate after 24 hrs by Bright-Glo luciferase reporter gene assay	[PMID: 29359565]
HEK293	IC₅₀	0.006 μM Compound: 41; (Z-LL)2	Inhibition of human SPPL2a expressed in HEK293 cells using GAL4-VP16 fusedTNFalpha (1 to 76)-NTF as substrate after 24 hrs by Bright-Glo luciferase reporter gene assay Inhibition of human SPPL2a expressed in HEK293 cells using GAL4-VP16 fusedTNFalpha (1 to 76)-NTF as substrate after 24 hrs by Bright-Glo luciferase reporter gene assay	[PMID: 29359565]
HEK293	IC₅₀	2 μM Compound: (Z-LL)2-ketone	Inhibition of SPP expressed in HEK293 cells in presence of Prl-PP peptide after 30 mins Inhibition of SPP expressed in HEK293 cells in presence of Prl-PP peptide after 30 mins	[PMID: 17932033]

Molecular Weight

809.00

Formula

C₄₃H₆₄N₆O₉

CAS No.

313664-40-3

Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (123.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2361 mL	6.1805 mL	12.3609 mL
5 mM	0.2472 mL	1.2361 mL	2.4722 mL
10 mM	0.1236 mL	0.6180 mL	1.2361 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.67%

Select Batch:

Data Sheet (268 KB) SDS (252 KB)

COA (244 KB) RP-HPLC (111 KB) MS (226 KB) Elemental Analysis Report (103 KB)

Handling Instructions (2659 KB)

References

[1]. A Weihofen, et al. Release of signal peptide fragments into the cytosol requires cleavage in the transmembrane region by a protease activity that is specifically blocked by a novel cysteine protease inhibitor. J Biol Chem. 2000 Oct 6;275(40):30951-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2361 mL	6.1805 mL	12.3609 mL	30.9023 mL
	5 mM	0.2472 mL	1.2361 mL	2.4722 mL	6.1805 mL
	10 mM	0.1236 mL	0.6180 mL	1.2361 mL	3.0902 mL
	15 mM	0.0824 mL	0.4120 mL	0.8241 mL	2.0602 mL
	20 mM	0.0618 mL	0.3090 mL	0.6180 mL	1.5451 mL
	25 mM	0.0494 mL	0.2472 mL	0.4944 mL	1.2361 mL
	30 mM	0.0412 mL	0.2060 mL	0.4120 mL	1.0301 mL
	40 mM	0.0309 mL	0.1545 mL	0.3090 mL	0.7726 mL
	50 mM	0.0247 mL	0.1236 mL	0.2472 mL	0.6180 mL
	60 mM	0.0206 mL	0.1030 mL	0.2060 mL	0.5150 mL
	80 mM	0.0155 mL	0.0773 mL	0.1545 mL	0.3863 mL
	100 mM	0.0124 mL	0.0618 mL	0.1236 mL	0.3090 mL

(Z-LL)2 ketone Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(Z-LL)2 ketone313664-40-3Ser/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesketoneSignal peptidesN-terminal fragmentInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

(Z-LL)2 ketone

Customer Review

(Z-LL)2 ketone Related Antibodies

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

(Z-LL)2 ketone Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report