Yuanhuacine (Synonyms: Gnidilatidin)

Name: Yuanhuacine
Brand: MedChemExpress
SKU: HY-N7225
Rating: 4.5 (1 reviews)

Cat. No.: HY-N7225 Purity: 95.89%: FAQs
Data Sheet SDS COA Handling Instructions

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Yuanhuacine (Gnidilatidin), a diterpene from Daphne genkwa, is an effective and highly selective inhibitor of the basal-like 2 (BL2) subtype of TNBC. Yuanhuacine can induce G2/M cell cycle arrest and has broad anti-tumor activity. Yuanhuacine is an orally active DNA damaging agent.

For research use only. We do not sell to patients.

Yuanhuacine Chemical Structure

CAS No. : 60195-70-2

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	13.11 μM Compound: 12	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 23558238]
B16	IC₅₀	> 30 μM Compound: 3	Cytotoxicity against alpha-MSH stimulated mouse B16 cells after 72 hrs by MTT assay Cytotoxicity against alpha-MSH stimulated mouse B16 cells after 72 hrs by MTT assay	[PMID: 23623417]
B16	IC₅₀	0.16 μM Compound: 3	Inhibition of melanin production in alpha-MSH stimulated mouse B16 cells after 72 hrs by spectrophotometry Inhibition of melanin production in alpha-MSH stimulated mouse B16 cells after 72 hrs by spectrophotometry	[PMID: 23623417]
HCT-116	IC₅₀	22.96 μM Compound: 12	Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 23558238]
HeLa	IC₅₀	5.56 μM Compound: 12	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 23558238]
HepG2	IC₅₀	8.04 μM Compound: 12	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 23558238]
HL-60	IC₅₀	26.81 μM Compound: 12	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 23558238]
HT-1080	IC₅₀	2.02 μM Compound: 12	Cytotoxicity against human HT1080 cells after 48 hrs by MTT assay Cytotoxicity against human HT1080 cells after 48 hrs by MTT assay	[PMID: 23558238]
K562	IC₅₀	16.08 μM Compound: 12	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 23558238]
MCF7	IC₅₀	2.39 μM Compound: 12	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 23558238]
MT4	IC₅₀	> 34 nM Compound: 4	Cytotoxicity against human MT4 cells assessed as reduction in cell viability incubated for 4 days by CellTiter-Glo assay Cytotoxicity against human MT4 cells assessed as reduction in cell viability incubated for 4 days by CellTiter-Glo assay	[PMID: 32997496]
MT4	CC₅₀	> 6.2 μM Compound: 24	Cytotoxicity against human MT4 cells incubated for 3 days by CellTiter-Glo luminescent assay Cytotoxicity against human MT4 cells incubated for 3 days by CellTiter-Glo luminescent assay	[PMID: 31860304]
RKO	IC₅₀	6.5 μM Compound: 12	Cytotoxicity against human RKO cells after 24 hrs by MTT assay Cytotoxicity against human RKO cells after 24 hrs by MTT assay	[PMID: 32223193]
SW-620	IC₅₀	4.3 μM Compound: 12	Cytotoxicity against human SW620 cells after 24 hrs by MTT assay Cytotoxicity against human SW620 cells after 24 hrs by MTT assay	[PMID: 32223193]
U-937	IC₅₀	11.79 μM Compound: 12	Cytotoxicity against human U937 cells after 48 hrs by MTT assay Cytotoxicity against human U937 cells after 48 hrs by MTT assay	[PMID: 23558238]

In Vitro

Yuanhuacine (0-100 μM, 24-72 h) inhibits the proliferation of H1993, H358, H460, Calu-1, H1299 and A549 cells, with IC₅₀ values of 0.009, 16.5, 6.2, 4.1, 4.0 and 0.03 μM^[1].
Yuanhuacine (0-1000 nM, 24 h) inhibits the p-AMPK protein levels in H1993 cells and downregulates the activation of mTOR^[1].
Yuanhuacine (10-100 nM, 0-24 h) inhibits the migration of H1993 cells^[1].
Yuanhuacine (0-16 μM, 24 h) inhibits the proliferation of UMUC3 and HCT116 cells, with IC₅₀ values of 1.89 and 14.28 μM^[2].
Yuanhuacine (2 μM, 12 h) induces G2/M phase arrest in T24T and HCT116 cells^[2].
Yuanhuacine (0-2 μM, 12 h) upregulates the transcription and protein expression of p21 by regulating the stability of Sp1 protein^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	H1993 cell
Concentration:	0, 0.00128, 0.0064, 20, 100 μM
Incubation Time:	24, 48, 72 h
Result:	Inhibited cell proliferation in a concentration- and time-dependent manner.

Western Blot Analysis^[1]

Cell Line:	H1993 cell
Concentration:	0, 10, 100, 500, 1000 nM
Incubation Time:	24 h
Result:	Significantly increased p-AMPK protein levels, decreased ACC levels, inhibited the autophosphorylation of mTOR at Ser2481 site, inhibited Akt, PKC-α, Rac1 and F-actin, and activated the cell adhesion molecule E-cadherin expression.

Cell Migration Assay ^[1]

Cell Line:	H1993 cell
Concentration:	10, 100 nM
Incubation Time:	0, 24 h
Result:	Inhibited cell migration in a concentration-dependent manner.

Cell Viability Assay^[2]

Cell Line:	T24T, UMUC3, HCT116
Concentration:	0, 2, 4, 8, 16 μM
Incubation Time:	24 h
Result:	Inhibited cell proliferation in a dose-dependent manner.

Cell Cycle Analysis^[2]

Cell Line:	T24T, HCT116
Concentration:	2 μM
Incubation Time:	12 h
Result:	Induced significant G2/M phase arrest.

Western Blot Analysis^[2]

Cell Line:	T24T, HCT116
Concentration:	0, 1, 2 μM
Incubation Time:	12 h
Result:	Up-regulated the expression of p21 protein, but did not show observable induction of p53 protein expression and p53 protein Ser-15 phosphorylation site.

In Vivo

Yuanhuacine (0.5-1 mg/kg, oral, once daily, 21 days) inhibits tumor proliferation in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	H1993 cells implanted xenograft mouse model^[1]
Dosage:	0.5, 1 mg/kg; daily; 21 days
Administration:	Oral
Result:	Inhibited tumor growth by 33.4% and 38.8%, respectively, reduced tumor weight, activated AMPK, and inhibited proliferation biomarkers Ki-67 and PCNA in tumor tissues.

Molecular Weight

648.74

Formula

C₃₇H₄₄O₁₀

CAS No.

60195-70-2

Appearance

Solid

Color

White to off-white

SMILES

O=C1C(C)=C[C@]2([H])[C@]1(O)[C@H](O)[C@]3(CO)[C@@]([C@]4([H])[C@]25[C@H](C)[C@@H](OC(C6=CC=CC=C6)=O)[C@@]7(C(C)=C)[C@]4([H])O[C@](O5)(/C=C/C=C/CCCCC)O7)([H])O3

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (154.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5414 mL	7.7072 mL	15.4145 mL
5 mM	0.3083 mL	1.5414 mL	3.0829 mL
10 mM	0.1541 mL	0.7707 mL	1.5414 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.47%

Select Batch:

Data Sheet (284 KB) SDS (252 KB)

COA (259 KB) HNMR (248 KB) CNMR (222 KB) RP-HPLC (301 KB) MS (1015 KB)

Handling Instructions (2659 KB)

References

[1]. Ji In Kang, et al. Anti-Tumor Activity of Yuanhuacine by Regulating AMPK/mTOR Signaling Pathway and Actin Cytoskeleton Organization in Non-Small Cell Lung Cancer Cells. PLoS One. 2015 Dec 11;10(12):e0144368. [Content Brief]

[2]. Ruowen Zhang, et al. The Chinese herb isolate yuanhuacine (YHL-14) induces G2/M arrest in human cancer cells by up-regulating p21 protein expression through an p53 protein-independent cascade. J Biol Chem. 2014 Mar 7;289(10):6394-403. [Content Brief]

[3]. Charles S Fermaintt, et al. Yuanhuacine Is a Potent and Selective Inhibitor of the Basal-Like 2 Subtype of Triple Negative Breast Cancer with Immunogenic Potential. Cancers (Basel). 2021 Jun 7;13(11):2834. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5414 mL	7.7072 mL	15.4145 mL	38.5362 mL
	5 mM	0.3083 mL	1.5414 mL	3.0829 mL	7.7072 mL
	10 mM	0.1541 mL	0.7707 mL	1.5414 mL	3.8536 mL
	15 mM	0.1028 mL	0.5138 mL	1.0276 mL	2.5691 mL
	20 mM	0.0771 mL	0.3854 mL	0.7707 mL	1.9268 mL
	25 mM	0.0617 mL	0.3083 mL	0.6166 mL	1.5414 mL
	30 mM	0.0514 mL	0.2569 mL	0.5138 mL	1.2845 mL
	40 mM	0.0385 mL	0.1927 mL	0.3854 mL	0.9634 mL
	50 mM	0.0308 mL	0.1541 mL	0.3083 mL	0.7707 mL
	60 mM	0.0257 mL	0.1285 mL	0.2569 mL	0.6423 mL
	80 mM	0.0193 mL	0.0963 mL	0.1927 mL	0.4817 mL
	100 mM	0.0154 mL	0.0771 mL	0.1541 mL	0.3854 mL

Yuanhuacine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Yuanhuacine60195-70-2GnidilatidinDNA/RNA SynthesisOrallyantiproliferativeanti-tumordaphnanediterpenoidInhibitorinhibitorinhibit

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Yuanhuacine (Synonyms: Gnidilatidin)

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