2. PROTAC Stem Cell/Wnt
  3. PROTACs β-catenin Wnt
  4. xStAx-VHLL TFA

xStAx-VHLL TFA is a β-catenin PROTAC degrader that promotes ubiquitination and proteasome degradation of β-catenin. xStAx-VHLL TFA inhibits the Wnt/β-catenin signaling pathway. xStAx-VHLL TFA can inhibit the proliferation of colon cancer cells and has anti-tumor activity.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

xStAx-VHLL TFA Chemical Structure

xStAx-VHLL TFA Chemical Structure

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Based on 1 publication(s) in Google Scholar

Other Forms of xStAx-VHLL TFA:

xStAx-VHLL TFA Related Antibodies

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Description

xStAx-VHLL TFA is a β-catenin PROTAC degrader that promotes ubiquitination and proteasome degradation of β-catenin. xStAx-VHLL TFA inhibits the Wnt/β-catenin signaling pathway. xStAx-VHLL TFA can inhibit the proliferation of colon cancer cells and has anti-tumor activity[1].

In Vitro

Non-salt dose:
xStAx-VHLL (10-60 μM; 1-6 days) induces β-catenin degradation in a dose-dependent manner in colorectal cancer cells and inhibits cell proliferation[1].
xStAx-VHLL (50-90 μM; 24 h) inhibits Wnt/β-catenin signaling in HEK293T cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

xStAx-VHLL TFA Related Antibodies

Western Blot Analysis[1]

Cell Line: HCT116 cells
Concentration: 10, 20, 30, 40, 50 and 60 μM
Incubation Time: 24 h
Result: Reduced the level of β-catenin in a dose-dependent manner.
In Vivo

Non-salt dose:
xStAx-VHLL (30 mg/kg; Intraperitoneal injection; 14 days) has antitumor activity in mouse intestinal tumor model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: APC min/+ mice that harbored a point mutation in the APC gene, resulting in APC deletion and constitutive activation of Wnt signaling (APC min/+ mice develops intestinal tumors around 10 weeks-old) [1]
Dosage: 30 mg/kg
Administration: Intraperitoneal injection (i.p.)； 14days
Result: Dramatically reduced tumor number.
Reduced the active of β-catenin protein levels.
Molecular Weight

3302.20

Formula

C140H222N46O27S10
Appearance

Solid

Color

White to off-white

Sequence

Ac-Arg-Arg-Trp-Pro-Arg-{S5}-Ile-Leu-Asp-{S5}-His-Val-Arg-Arg-Val-Trp-Arg-{Ahx}-Ala-Leu-Ala-Pro-Tyr-Ile-Pro-NH2
Sequence Shortening

Ac-RRWPR{S5}ILD{S5}HVRRVWR-{Ahx}-ALAPYIP-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (30.28 mM; Need ultrasonic)

DMSO : 100 mg/mL (30.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.3028 mL 1.5141 mL 3.0283 mL
5 mM 0.0606 mL 0.3028 mL 0.6057 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.22%

SDS (252 KB)

COA (255 KB) RP-HPLC (111 KB) MS (294 KB) Elemental Analysis Report (114 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 0.3028 mL 1.5141 mL 3.0283 mL 7.5707 mL
5 mM 0.0606 mL 0.3028 mL 0.6057 mL 1.5141 mL
10 mM 0.0303 mL 0.1514 mL 0.3028 mL 0.7571 mL
15 mM 0.0202 mL 0.1009 mL 0.2019 mL 0.5047 mL
20 mM 0.0151 mL 0.0757 mL 0.1514 mL 0.3785 mL
25 mM 0.0121 mL 0.0606 mL 0.1211 mL 0.3028 mL
30 mM 0.0101 mL 0.0505 mL 0.1009 mL 0.2524 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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xStAx-VHLL TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

xStAx-VHLLPROTACsβ-cateninWntBeta catenincolorectal cancerHCT116 cellsHEK293T cellsInhibitorinhibitorinhibit

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