2. Stem Cell/Wnt Apoptosis Cell Cycle/DNA Damage
  3. Deubiquitinase YAP MDM-2/p53 CDK Apoptosis
  4. USP10-IN-4

USP10-IN-4 is a potent ubiquitin-specific protease 10 (USP10) inhibitor with an IC50 of 10.87 μM and a Kd of 365 nM. USP10-IN-4 effectively inhibits USP10-mediated proteasomal degradation of downstream proteins YAP and p53, leading to the subsequent downregulation of CDK4 in the p53 signaling pathway. USP10-IN-4 promotes apoptosis in HCC cells and inhibits the onset and progression of liver cancer. USP10-IN-4 can used for the study of hepatocellular carcinoma (HCC).

For research use only. We do not sell to patients.

USP10-IN-4

USP10-IN-4 Chemical Structure

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USP10-IN-4 Related Antibodies

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Description

USP10-IN-4 is a potent ubiquitin-specific protease 10 (USP10) inhibitor with an IC50 of 10.87 μM and a Kd of 365 nM. USP10-IN-4 effectively inhibits USP10-mediated proteasomal degradation of downstream proteins YAP and p53, leading to the subsequent downregulation of CDK4 in the p53 signaling pathway. USP10-IN-4 promotes apoptosis in HCC cells and inhibits the onset and progression of liver cancer. USP10-IN-4 can used for the study of hepatocellular carcinoma (HCC)[1].

IC50 & Target[1]

CDK4

 

In Vitro

USP10-IN-4 (Compound LY-2) (0.1-10 μM) inhibits the proliferation of Huh-7 and Bel-7402 cells in a concentration-dependent manner with IC50s of 2.5 and 1.3 μM[1].
USP10-IN-4 (1-20 μM, 14 d) consistently inhibits Huh-7 cells colony formation[1].
USP10-IN-4 (5-40 μM, 24 h) induces apoptosis and G1 arrest in Huh-7 cells[1].
USP10-IN-4 (0-20 μM, 0-48 h) results in the decrease of YAP, p53, CDK4 and Cyclin B2 protein in Huh-7 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

USP10-IN-4 Related Antibodies

Cell Cycle Analysis[1]

Cell Line: Huh-7 cells
Concentration: 5, 20 and 40 μM
Incubation Time: 24 h
Result: Increased the proportion of cells in the G1 phase from 45% to 68%.

Western Blot Analysis[1]

Cell Line: Huh-7 cells
Concentration: 0, 0.5, 1, 5, 10 and 20 μM
Incubation Time: 0, 6, 12, 24, 36, 48 h
Result: Reduced the expression of YAP and p53 in a time- and concentration-dependent manner.
Reduced CDK4, and Cyclin B2 proteins.
Molecular Weight

487.98

Formula

C28H26ClN3O3
SMILES

O=C(C(N(CC(NCCC1=CC=C(Cl)C=C1)=O)C2=C3C=CC(C)=C2)=CC3=O)NC4=CC=CC=C4C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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USP10-IN-4 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

USP10-IN-4DeubiquitinaseYAPMDM-2/p53CDKApoptosisDUBsYes-associated proteinCyclin dependent kinaseHCCDeubiquitinationBinding affinityCDK4Inhibitorinhibitorinhibit

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