Tubulin inhibitor 24

Name: Tubulin inhibitor 24
Brand: MedChemExpress
SKU: HY-146711
Rating: 4.5 (1 reviews)

Cat. No.: HY-146711 Purity: 99.94%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Tubulin inhibitor 24 is a potent tubulin inhibitor. Tubulin inhibitor 24 inhibits tubulin polymerization. Tubulin inhibitor 24 induces cell cycle arrest at the G2/M phase in a concentration-dependent manner. Tubulin inhibitor 24 shows antitumor activity with no obvious toxicity.

For research use only. We do not sell to patients.

Tubulin inhibitor 24 Chemical Structure

CAS No. : 2415761-65-6

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
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10 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.003 μM Compound: 1b	Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay	[PMID: 32650183]
B16-F0	IC₅₀	0.021 μM Compound: 1b	Antiproliferative activity against mouse B16-F0 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay Antiproliferative activity against mouse B16-F0 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay	[PMID: 32650183]
HCT-116	IC₅₀	0.048 μM Compound: 1b	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay	[PMID: 32650183]
HeLa	IC₅₀	0.021 μM Compound: 1b	Antiproliferative activity against human HeLa cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay	[PMID: 32650183]
MCF7	IC₅₀	0.047 μM Compound: 1b	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay	[PMID: 32650183]

In Vitro

Tubulin inhibitor 24 (compound 1b) () shows high antiproliferative activity with IC₅₀s of 0.021, 0.047, 0.003, 0.048 µM for Hela, MCF-7, A549, HCT-116, B16-F10 cells, respectively^[1].
Tubulin inhibitor 24 inhibites tubulin polymerization with an IC₅₀ value of 2.1 µM^[1].
Tubulin inhibitor 24 (5, 10 nM) induces cell cycle arrest at the G2/M phase in a concentration-dependent manner^[1].
Tubulin inhibitor 24 (10, 20, 40 nM; 24 h) inhibits MCF-7 cancer cells migration in a dose-dependent manner^[1].
Tubulin inhibitor 24 (40 nM; 6 h) destabilizes microtubule by inhibiting tubulin polymerization and disturbing microtubule networks in B16-F10 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Hela, MCF-7, A549, HCT-116, B16-F10 cells
Concentration:	0.00098, 0.0039, 0.016, 0.0625, 0.25, 1.0, 4.0, 16, 64 µM
Incubation Time:	48 h
Result:	Showed high antiproliferative activity with IC₅₀s of 0.021, 0.047, 0.003, 0.048 µM for Hela, MCF-7, A549, HCT-116, B16-F10 cells, respectively.

Cell Cycle Analysis^[1]

Cell Line:	MCF-7 cells
Concentration:	5, 10 nM
Incubation Time:	48 h
Result:	Cells were arrested at the G2/M phase in a concentration-dependent manner.

In Vivo

Tubulin inhibitor 24 (10, 20 mg/kg; i.p.; per day for 16 days) shows antitumor activity with no obvious toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	4-6 weeks, male C57/BL mice (B16e-10 tumor model)^[1]
Dosage:	10, 20 mg/kg (formulated in 5% DMSO, 40% PEG-300 and 55% saline)
Administration:	I.p.; per day, 16 days
Result:	Showed antitumor activity with no obvious toxicity.

Molecular Weight

375.42

Formula

C₂₂H₂₁N₃O₃

CAS No.

2415761-65-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

COC1=CC(C2=CC=NC3=CC(C4=CC=C(C=C4)C)=NN32)=CC(OC)=C1OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 6.25 mg/mL (16.65 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6637 mL	13.3184 mL	26.6368 mL
5 mM	0.5327 mL	2.6637 mL	5.3274 mL
10 mM	0.2664 mL	1.3318 mL	2.6637 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.94%

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Data Sheet (275 KB) SDS (251 KB)

COA (251 KB) HNMR (144 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. Li G,et al. Design, synthesis, and bioevaluation of pyrazolo[1,5-a]pyrimidine derivatives as tubulin polymerization inhibitors targeting the colchicine binding site with potent anticancer activities. Eur J Med Chem. 2020; 202:112519. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6637 mL	13.3184 mL	26.6368 mL	66.5921 mL
	5 mM	0.5327 mL	2.6637 mL	5.3274 mL	13.3184 mL
	10 mM	0.2664 mL	1.3318 mL	2.6637 mL	6.6592 mL
	15 mM	0.1776 mL	0.8879 mL	1.7758 mL	4.4395 mL