Tubulin-IN-53 

Tubulin-IN-53 is a potent Tubulin inhibitor with an IC₅₀ of 6.06 μM. Tubulin-IN-53 inhibits the polymerization of tubulin by targeting the colchicine binding site of tubulin and destroys the microtubule network. Tubulin-IN-53 induces MCF-7 cell cycle arrest in the G2/M phase and apoptosis, and inhibits cell migration accompanied by the decrease of mitochondrial membrane potential and increase the accumulation of ROS. Tubulin-IN-53 destroys the angiogenesis of human umbilical vein endothelial cells.Tubulin-IN-53 can used for the study of cancers such as breast cancer and lung cancer^[1].

Tubulin-IN-53 (Compound A7) (72 h) exhibits antiproliferative activities in various cancer cells with IC₅₀s of 6, 4, 5, 6, 4 and 10 nM against PC-3, MCF-7, MDA-MB-231, A549, HT-29 and A549/TxR cells and cytotoxicity on normal cells with CC₅₀s of 112 and 220 nM against L02 and MCF-10A cells^[1].
Tubulin-IN-53 (5-20 nM, 14 d) consistently inhibits MCF-7 cells colony formation^[1].
Tubulin-IN-53 (5-20 nM, 6 h) dose-dependently inhibits the the formation of EBI and β-tubulin adducts and completely disaggregats the microtubule network in MCF-7 cells^[1].
Tubulin-IN-53 (5-20 nM, 24 h) induces MCF-7 cell cycle arrest in the G2/M phase and cell apoptosis, and significantly increases intracellular ROS levels^[1].
Tubulin-IN-53 (2.5-10 nM, 24 h) inhibits A549 cell migration and effectively inhibits the formation of vascular networks in HUVECs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

401.80

C₂₀H₁₆ClNO₆

O=C1OCC2=C1C(C3=CC(OC)=C(OC)C(Cl)=C3)C4=CC5=C(OCO5)C=C4N2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zhang M, et al. Design, synthesis and biological activity of tubulin inhibitors based on the structure of deoxypodophyllotoxin. Bioorg Chem. 2025 Aug;163:108783.  [Content Brief]