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  3. THAM hydrochloride (≥99%, for cell culture)

THAM hydrochloride (≥99%, for cell culture)  (Synonyms: Tris HCl (≥99%, for cell culture); Tris hydrochloride (≥99%, for cell culture))

Cat. No.: HY-D0227J Purity: 99.67%
Handling Instructions Technical Support

THAM hydrochloride (≥99%, for cell culture) is a biochemical reagent.

For research use only. We do not sell to patients.

THAM hydrochloride (≥99%, for cell culture) Chemical Structure

THAM hydrochloride (≥99%, for cell culture) Chemical Structure

CAS No. : 1185-53-1

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Based on 1 publication(s) in Google Scholar

Other Forms of THAM hydrochloride (≥99%, for cell culture):

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Description

THAM hydrochloride (≥99%, for cell culture) is a biochemical reagent.

Molecular Weight

157.60

Formula

C4H12ClNO3

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OCC(CO)(N)CO.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 35 mg/mL (222.08 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.3452 mL 31.7259 mL 63.4518 mL
5 mM 1.2690 mL 6.3452 mL 12.6904 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.3452 mL 31.7259 mL 63.4518 mL 158.6294 mL
5 mM 1.2690 mL 6.3452 mL 12.6904 mL 31.7259 mL
10 mM 0.6345 mL 3.1726 mL 6.3452 mL 15.8629 mL
15 mM 0.4230 mL 2.1151 mL 4.2301 mL 10.5753 mL
20 mM 0.3173 mL 1.5863 mL 3.1726 mL 7.9315 mL
25 mM 0.2538 mL 1.2690 mL 2.5381 mL 6.3452 mL
30 mM 0.2115 mL 1.0575 mL 2.1151 mL 5.2876 mL
40 mM 0.1586 mL 0.7931 mL 1.5863 mL 3.9657 mL
50 mM 0.1269 mL 0.6345 mL 1.2690 mL 3.1726 mL
60 mM 0.1058 mL 0.5288 mL 1.0575 mL 2.6438 mL
80 mM 0.0793 mL 0.3966 mL 0.7931 mL 1.9829 mL
100 mM 0.0635 mL 0.3173 mL 0.6345 mL 1.5863 mL
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THAM hydrochloride (≥99%, for cell culture) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
THAM hydrochloride (≥99%, for cell culture)
Cat. No.:
HY-D0227J
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