Tetrazine-SS-PEG4-Biotin

Name: Tetrazine-SS-PEG4-Biotin
Brand: MedChemExpress
SKU: HY-136036
Rating: 4.5 (1 reviews)

Cat. No.: HY-136036 Purity: 99.42%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Tetrazine-SS-PEG4-Biotin is a cleavable 4 unit PEG ADC linker used in the synthesis of antibody-drug conjugates (ADCs). Tetrazine-SS-PEG4-Biotin is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

For research use only. We do not sell to patients.

Tetrazine-SS-PEG4-Biotin Chemical Structure

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	Get quote	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Tetrazine-SS-PEG4-Biotin is a cleavable 4 unit PEG ADC linker used in the synthesis of antibody-drug conjugates (ADCs)^[1]. Tetrazine-SS-PEG4-Biotin is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

IC₅₀ & Target

Disulfide Cleavable Linker

Cleavable Linker

In Vitro

ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

824.05

Formula

C₃₅H₅₃N₉O₈S₃

Appearance

Solid

Color

Purple to purplish red

SMILES

O=C(CCSSCCNC(CCOCCOCCOCCOCCNC(CCCC[C@@H]1SC[C@H](N2)[C@@H]1NC2=O)=O)=O)NCC(C=C3)=CC=C3C4=NN=CN=N4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (60.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2135 mL	6.0676 mL	12.1352 mL
5 mM	0.2427 mL	1.2135 mL	2.4270 mL
10 mM	0.1214 mL	0.6068 mL	1.2135 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.42%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (258 KB) HNMR (266 KB) RP-HPLC (230 KB) LCMS (133 KB)

Handling Instructions (2659 KB)

References

[1]. Beck A, et al. Strategies and challenges for the next generation of antibody-drug conjugates. Nat Rev Drug Discov. 2017 May;16(5):315-337. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2135 mL	6.0676 mL	12.1352 mL	30.3380 mL
	5 mM	0.2427 mL	1.2135 mL	2.4270 mL	6.0676 mL
	10 mM	0.1214 mL	0.6068 mL	1.2135 mL	3.0338 mL
	15 mM	0.0809 mL	0.4045 mL	0.8090 mL	2.0225 mL
	20 mM	0.0607 mL	0.3034 mL	0.6068 mL	1.5169 mL
	25 mM	0.0485 mL	0.2427 mL	0.4854 mL	1.2135 mL
	30 mM	0.0405 mL	0.2023 mL	0.4045 mL	1.0113 mL
	40 mM	0.0303 mL	0.1517 mL	0.3034 mL	0.7584 mL
	50 mM	0.0243 mL	0.1214 mL	0.2427 mL	0.6068 mL
	60 mM	0.0202 mL	0.1011 mL	0.2023 mL	0.5056 mL