2. Apoptosis
  3. Apoptosis
  4. Tat-NTS peptide

Tat-NTS peptide is a cell-penetrating peptide with neuroprotective effects. Tat-NTS peptide can specifically inhibit the nuclear translocation of ANXA1 and reduce neuronal apoptosis in ischemic areas. Moreover, Tat-NTS peptide can reduce the volume of cerebral ischemic infarction and can be used in the research of ischemic stroke.

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Tat-NTS peptide

Tat-NTS peptide Chemical Structure

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Based on 1 publication(s) in Google Scholar

Tat-NTS peptide Related Antibodies

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Description

Tat-NTS peptide is a cell-penetrating peptide with neuroprotective effects. Tat-NTS peptide can specifically inhibit the nuclear translocation of ANXA1 and reduce neuronal apoptosis in ischemic areas. Moreover, Tat-NTS peptide can reduce the volume of cerebral ischemic infarction and can be used in the research of ischemic stroke[1].

In Vitro

Tat-NTS peptide (20 μM) blocks ANXA1 nuclear migration in neuronal cells without affecting the nuclear translocation of other proteins[1].
Tat-NTS peptide (20 μM) inhibits the binding of ANXA1 to p53, alleviates neuronal apoptosis induced by oxygen glucose deprivation/reperfusion (OGD/R), and has neuronal cytoprotective effects[1].
Tat-NTS peptide (20 μM) can significantly improve the viability of neuronal cells after OGD/R[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tat-NTS peptide Related Antibodies

Western Blot Analysis[1]

Cell Line: neuronal cells
Concentration: 20 μM
Incubation Time:
Result: Significantly decreased the level of ANXA1 protein in the nuclear fraction, and had no impact on the subcellular localization of p65 and β-catenin.
Significantly decreased Bid protein levels.
Significantly decreased OGD/R-triggered tBid expression and caspase-9, PARP, caspase-3 cleavage but had little effect on the activation of caspase-8.

RT-PCR[1]

Cell Line: neuronal cells
Concentration: 20 μM
Incubation Time:
Result: Significantly decreased Bid mRNA levels.
In Vivo

Tat-NTS peptide (0.5-10 mg/kg; nilateral Intracerebroventricular (i.c.v.) Injection; single dose) reduces the interaction between ANXA1 and importin β in C57BL/6 mice with middle cerebral artery occlusion (MCAO) and prevents the nuclear translocation of ANXA1 in vivo. Tat-NTS peptide alleviates neuronal apoptosis after ischemic injury in mice, exhibiting neuroprotective effects against cerebral ischemia[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MCAO C57BL/6 mice model[1]
Dosage: 0.5 mg/kg, 1 mg/kg, 2 mg/kg, 5 mg/kg, 10 mg/kg.
Administration: Unilateral Intracerebroventricular (i.c.v.) Injection: Single dose. After undergoing 1-hour middle cerebral artery occlusion (MCAO)
Result: Significantly inhibited the protein level of tBid, cleaved caspase-9, cleaved PARP, and caspase-3, and had little effect on the activation of caspase-8.
Reduced the infarct volume and improved neurological outcomes following focal ischemic injury.
Molecular Weight

2855.37

Formula

C125H212N54O24
Appearance

Solid

Sequence

Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Arg-Ser-Phe-Pro-His-Leu-Arg-Arg-Val-Phe-NH2
Sequence Shortening

YGRKKRRQRRRRSFPHLRRVF-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (35.02 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.3502 mL 1.7511 mL 3.5022 mL
5 mM 0.0700 mL 0.3502 mL 0.7004 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.3502 mL 1.7511 mL 3.5022 mL 8.7554 mL
5 mM 0.0700 mL 0.3502 mL 0.7004 mL 1.7511 mL
10 mM 0.0350 mL 0.1751 mL 0.3502 mL 0.8755 mL
15 mM 0.0233 mL 0.1167 mL 0.2335 mL 0.5837 mL
20 mM 0.0175 mL 0.0876 mL 0.1751 mL 0.4378 mL
25 mM 0.0140 mL 0.0700 mL 0.1401 mL 0.3502 mL
30 mM 0.0117 mL 0.0584 mL 0.1167 mL 0.2918 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Tat-NTS peptide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tat-NTS peptideApoptosisC57BL/6 miceneuronal apoptosisoxygen glucose deprivation/reperfusion (OGD/R)middle cerebral artery occlusion (MCAO)neuroprotectantischemic strokeInhibitorinhibitorinhibit

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