Taq DNA polymerase 

Taq DNA polymerase is a thermostable DNA polymerase that can be used in PCR^[1].

Product Information
This product is produced by modifying the protein molecule of wild-type Taq DNA polymerase and expressing it in E. coli to enhance the enzyme's anti-interference ability.
The storage buffer: 20 mM Tris, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% NP-40, 0.5% Tween-20, 50% Glycerol, pH8.0.
Reaction buffer: 100 mM Tris, 500 mM KCl, 15 mM MgCl2, 0.8% NP-40, pH 8.8

PCR amplification directly from whole blood
1. PCR system
2.5 μL reaction buffer, up to 5 μL whole blood DNA template, 2.5 μL 2.5 mM dNTP, 1 μL upstream primer (10 μM), 1 μL downstream primer (10 μM), 0.5 μL Taq DNA polymerase, and Nuclease-free water to make up the volume to 25 μL.
2. PCR cycle
1) 95℃ 3min cycle 1 time;
2) 94℃ 30sec, 55℃ 30sec, 72℃ 45-90sec, cycle 35-40 times;
3) 72℃ 7min cycle 1 time;

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2304873-37-6

Liquid

Colorless to light yellow

[Taq DNA polymerase]

≥50 U/μL

One unit is defined as the amount of enzyme that catalyze the incorporation of 1 nmol AMP into the polynucleotide at 37°C in 60 min.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zhou YH, et al. Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase. Nucleic Acids Res. 1991 Nov 11;19(21):6052.  [Content Brief]