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  3. SYBR green I chloride

SYBR green I (chloride) is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.

For research use only. We do not sell to patients.

SYBR green I chloride

SYBR green I chloride Chemical Structure

CAS No. : 2225748-05-8

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Based on 1 publication(s) in Google Scholar

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Description

SYBR green I (chloride) is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.

Molecular Weight

545.18

Formula

C32H37ClN4S

CAS No.
Appearance

Solid

Color

Orange to red

SMILES

CCCN(CCCN(C)C)C(C=C1/C=C2N(C)C3=C(C=CC=C3)S\2)=[N+](C4=C1C=CC=C4)C5=CC=CC=C5.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (91.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8343 mL 9.1713 mL 18.3426 mL
5 mM 0.3669 mL 1.8343 mL 3.6685 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8343 mL 9.1713 mL 18.3426 mL 45.8564 mL
5 mM 0.3669 mL 1.8343 mL 3.6685 mL 9.1713 mL
10 mM 0.1834 mL 0.9171 mL 1.8343 mL 4.5856 mL
15 mM 0.1223 mL 0.6114 mL 1.2228 mL 3.0571 mL
20 mM 0.0917 mL 0.4586 mL 0.9171 mL 2.2928 mL
25 mM 0.0734 mL 0.3669 mL 0.7337 mL 1.8343 mL
30 mM 0.0611 mL 0.3057 mL 0.6114 mL 1.5285 mL
40 mM 0.0459 mL 0.2293 mL 0.4586 mL 1.1464 mL
50 mM 0.0367 mL 0.1834 mL 0.3669 mL 0.9171 mL
60 mM 0.0306 mL 0.1529 mL 0.3057 mL 0.7643 mL
80 mM 0.0229 mL 0.1146 mL 0.2293 mL 0.5732 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
SYBR green I chloride
Cat. No.:
HY-D1191
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