2. Metabolic Enzyme/Protease Others
  3. Glycosidase Fluorescent Dye
  4. SPiDER-βGal-2

SPiDER-βGal-2 is a fluorogenic β-galactosidase substrate and suitable for labeling live cells in culture and in living tissues. SPiDER-βGal-2 can generate quinone methide through enzymatic reaction and form covalent bonds with nucleophilic functional groups of proteins near the molecule, so that the fluorescent product is fixed in living cells (Ex/Em = 493, 525 nm/560 nm).

For research use only. We do not sell to patients.

SPiDER-βGal-2 Chemical Structure

SPiDER-βGal-2 Chemical Structure

CAS No. : 1824699-55-9

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Based on 1 publication(s) in Google Scholar

SPiDER-βGal-2 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

SPiDER-βGal-2 is a fluorogenic β-galactosidase substrate and suitable for labeling live cells in culture and in living tissues. SPiDER-βGal-2 can generate quinone methide through enzymatic reaction and form covalent bonds with nucleophilic functional groups of proteins near the molecule, so that the fluorescent product is fixed in living cells (Ex/Em = 493, 525 nm/560 nm)[1][2].

In Vitro

1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 1 mM (optimized according to the experiment).
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1 µM with Hanks’ HEPES buffer (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Prepare cells for the assay.
2.2 Discard the culture medium and wash the cells with Hanks’ HEPES buffer twice.
3. Add SPiDER-βGal-2 (1 µM) working solution.
4. Incubate at 37°C for 15 minutes (optimized according to the experiment).
5. Observe the cells under a fluorescence microscope or by a flow cytometer (Ex/Em = 500-540/560 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SPiDER-βGal-2 Related Antibodies
Molecular Weight

585.59

Formula

C31H33F2NO8
CAS No.
Appearance

Solid

Color

Pink to orange

SMILES

FC(C1=C2C(C3(C4=CC=CC=C4CO3)C5=CC=C(N(CC)CC)C=C5O2)=CC=C1O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (170.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7077 mL 8.5384 mL 17.0768 mL
5 mM 0.3415 mL 1.7077 mL 3.4154 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7077 mL 8.5384 mL 17.0768 mL 42.6920 mL
5 mM 0.3415 mL 1.7077 mL 3.4154 mL 8.5384 mL
10 mM 0.1708 mL 0.8538 mL 1.7077 mL 4.2692 mL
15 mM 0.1138 mL 0.5692 mL 1.1385 mL 2.8461 mL
20 mM 0.0854 mL 0.4269 mL 0.8538 mL 2.1346 mL
25 mM 0.0683 mL 0.3415 mL 0.6831 mL 1.7077 mL
30 mM 0.0569 mL 0.2846 mL 0.5692 mL 1.4231 mL
40 mM 0.0427 mL 0.2135 mL 0.4269 mL 1.0673 mL
50 mM 0.0342 mL 0.1708 mL 0.3415 mL 0.8538 mL
60 mM 0.0285 mL 0.1423 mL 0.2846 mL 0.7115 mL
80 mM 0.0213 mL 0.1067 mL 0.2135 mL 0.5336 mL
100 mM 0.0171 mL 0.0854 mL 0.1708 mL 0.4269 mL
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SPiDER-βGal-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SPiDER-βGal-21824699-55-9GlycosidaseFluorescent Dyefluorogenic β-galactosidase substrate live cellsInhibitorinhibitorinhibit

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