2. GPCR/G Protein MAPK/ERK Pathway Cell Cycle/DNA Damage PROTAC
  3. SOS1 Ras PERK Ligands for Target Protein for PROTAC
  4. VUBI1

VUBI1 (SOS1 activator 1) is a benzimidazole derivative and SOS1 activator with a Kd of 44 nM. VUBI1 can significantly activate RAS-GTP and regulate the phosphorylation of ERK. VUBI1 also can serve as a target ligand for synthesizing PROTACs, such as PROTAC SOS1 degrader-1 (HY-145737), to induce SOS1 degradation. VUBI1 can be used in the study of cancer.

For research use only. We do not sell to patients.

The VUBI1 was designed by Boehringer Ingelheim and could be obtained free of charge through the Boehringer Ingelheim open innovation portal opnMe.com, associated with its negative control.

VUBI1 Chemical Structure

VUBI1 Chemical Structure

CAS No. : 2245237-53-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

VUBI1 Related Antibodies

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Description

VUBI1 (SOS1 activator 1) is a benzimidazole derivative and SOS1 activator with a Kd of 44 nM. VUBI1 can significantly activate RAS-GTP and regulate the phosphorylation of ERK. VUBI1 also can serve as a target ligand for synthesizing PROTACs, such as PROTAC SOS1 degrader-1 (HY-145737), to induce SOS1 degradation. VUBI1 can be used in the study of cancer[1][2][3][4].

In Vitro

VUBI1 (Compound 64) (0.3-30 µM; 10 min) increases RAS-GTP levels and biphasically modulates ERK1/2 phosphorylation in HeLa cells (inducing peak levels at low concentrations and suppressing at high concentrations)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

VUBI1 Related Antibodies

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 0.3, 1, 3, 10 and 30 µM
Incubation Time: 10 min
Result: Increased the level of RAS-GTP in a dose-dependent manner.
Increased the level of pERK1/2T202/Y204 at doses of 0.1 and 1 µM.
Molecular Weight

483.02

Formula

C26H32ClFN6
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

CN1CCN(C2=C3C(N(CC4=CC(C)=C(F)C(C)=C4)C(N5CC6(CNC6)C5)=N3)=CC(Cl)=C2)CC1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (20.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0703 mL 10.3515 mL 20.7031 mL
5 mM 0.4141 mL 2.0703 mL 4.1406 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0703 mL 10.3515 mL 20.7031 mL 51.7577 mL
5 mM 0.4141 mL 2.0703 mL 4.1406 mL 10.3515 mL
10 mM 0.2070 mL 1.0352 mL 2.0703 mL 5.1758 mL
15 mM 0.1380 mL 0.6901 mL 1.3802 mL 3.4505 mL
20 mM 0.1035 mL 0.5176 mL 1.0352 mL 2.5879 mL
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VUBI1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

VUBI12245237-53-8SOS1 activator 1VUBI 1VUBI-1SOS1RasPERKLigands for Target Protein for PROTACRas/Rac guanine nucleotide exchange factor 1Protein kinase R-like endoplasmic reticulum kinasePKR-like endoplasmic reticulum kinaseTarget Protein-binding MoietyHeLa cellsprotactarget ligandInhibitorinhibitorinhibit

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