Sodium thiosulfate (99%, water≤1.0%)  (Synonyms: Sodium hyposulfite (99%, water≤1.0%))

Sodium thiosulfate is an antioxidant. Sodium thiosulfate inhibits the expression of p-GSK-3β and β-catenin proteins, reduces IL-1β, COX-2, and Iba-1, and inhibits NFκB activation. Sodium thiosulfate promotes angiogenesis, inhibits inflammation, and improves acute lung injury. Sodium thiosulfate also exhibits anti-cancer activity against melanoma. Sodium thiosulfate also exerts renal protective effects. Sodium thiosulfate can be used in the research of osteoarthritis, brain inflammation, cancer (such as breast cancer, melanoma), and kidney disease^{[1][2][3][4][5][6][7][8][9]}.

Sodium thiosulfate (1.2-120 μM; 48 h) does not affect MCF-7 viability^[1].
Sodium thiosulfate (3 mM; 8 h) stimulates proliferation of human umbilical vein endothelial cells (HUVEC)^[2].
Sodium thiosulfate (0.2-25 mM; 1-7 days) dose-dependently inhibits mineralization of murine joint chondrocytes^[3].
Sodium thiosulfate (1-500 μM; 8 h) reduces LPS/IFNγ-induced release of TNFα and IL-6 in microglia^[4].
Sodium thiosulfate (0.1-10 mM; 48 h) does not attenuate the anticancer activity of Doxorubicin (HY-15142A) in human breast cancer MCF-7 cells and human cervical cancer HeLa cells^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sodium thiosulfate (0.5-1 g/kg/day; p.o. via water bottle; changed three times a week) promotes hindlimb ischemia-induced revascularization and muscle recovery in WT C57BL/6JRj mice and hypercholesterolemic LDLR^-/- mice, increases capillary density, reduces muscle damage^[2].
Sodium thiosulfate (0.4 g/kg; i.p.; three times per week) reduces the volume and crystal content of new periarticular calcific deposits, attenuates tibial and femoral cartilage damage and proteoglycan loss, and shows a significant positive correlation between the volume of new mineralized structures and tibial cartilage degradation score in menisectomy-induced osteoarthritis model of female C57BL/6 mice^[3].
Sodium thiosulfate (2 g/kg; intraperitoneal injection, 0 and 12 h after LPS challenge; 0.5 g/kg; i.v., 10 min after CLP) attenuates LPS or CLP-induced acute lung injury in male C57BL6J mice by inhibiting lung inflammation, reducing lung permeability, and suppressing NFκB activation^[5].
Sodium thiosulfate (2-3 g/kg; once daily) inhibits tumor growth and EMT process by the Wnt/β-catenin signaling pathway in nude mice xenografted with B16 cells^[8].
Sodium thiosulfate (100-750 mg/kg; i.p.) reduces brain inflammation induced by systemic lipopolysaccharide administration in female C57BL/6J mice by decreasing IL-1β, COX-2, Iba-1, and TSPO levels ^[9].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

158.11

Na₂S₂O₃

7772-98-7

Solid

White to off-white

[Na][Na].O=S(=O)=S=O

Room temperature in continental US; may vary elsewhere.

Store at room temperature, keep dry and cool

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Ota A, et al. The selective cytotoxicity of silver thiosulfate, a silver complex, on MCF-7 breast cancer cells through ROS-induced cell death. Pharmacol Rep. 2021 Jun;73(3):847-857.  [Content Brief]

  [2]. Macabrey D, et al. Sodium thiosulfate, a source of hydrogen sulfide, stimulates endothelial cell proliferation and neovascularization. Front Cardiovasc Med. 2022 Oct 3;9:965965.  [Content Brief]

  [3]. Nasi S, et al. Sodium Thiosulfate Prevents Chondrocyte Mineralization and Reduces the Severity of Murine Osteoarthritis. PLoS One. 2016 Jul 8;11(7):e0158196.  [Content Brief]

  [4]. Lee M, et al. Sodium thiosulfate attenuates glial-mediated neuroinflammation in degenerative neurological diseases. J Neuroinflammation. 2016 Feb 8;13:32.  [Content Brief]

  [5]. Sakaguchi M, et al. Sodium thiosulfate attenuates acute lung injury in mice. Anesthesiology. 2014 Dec;121(6):1248-57.  [Content Brief]

  [6]. Mizuta Y, et al. Sodium thiosulfate prevents doxorubicin-induced DNA damage and apoptosis in cardiomyocytes in mice. Life Sci. 2020 Sep 15;257:118074.  [Content Brief]

  [7]. Bijarnia RK, et al. Sodium thiosulfate ameliorates oxidative stress and preserves renal function in hyperoxaluric rats. PLoS One. 2015 Apr 30;10(4):e0124881.  [Content Brief]

  [8]. Wang D, et al. Sodium thiosulfate inhibits epithelial-mesenchymal transition in melanoma via regulating the Wnt/β-catenin signaling pathway. J Dermatol Sci. 2023 Feb;109(2):89-98.  [Content Brief]

  [9]. Acero G, et al. Sodium thiosulphate attenuates brain inflammation induced by systemic lipopolysaccharide administration in C57BL/6J mice. Inflammopharmacology. 2017 May 19.  [Content Brief]