SMD-3040 TFA

Cat. No.: HY-156568A Purity: 99.90%: Data Sheet
SDS

COA
Handling Instructions
FAQs
Technical Support

SMD-3040 TFA is a potent and selective SMARCA2 PROTAC degrader (DC₅₀: 12 nM; D_max: 91%). SMD-3040 TFA can inhibit tumor cell proliferation and exhibits antitumor activity. SMD-3040 TFA can be used in the study of tumors such as melanoma. (SMARCA2/4-ligand (HY-171765); HY-112078 VHL ligand (HY-112078))

For research use only. We do not sell to patients.

SMD-3040 TFA Chemical Structure

Size	Price	Stock
5 mg		Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of SMD-3040 TFA:

SMD-3040 SMD-3040 formate

Other Forms of SMD-3040 TFA:

SMD-3040 In-stock
SMD-3040 formate In-stock

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von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

Biological Activity
Purity & Documentation
References
Customer Review

Description

SMD-3040 TFA is a potent and selective SMARCA2 PROTAC degrader (DC₅₀: 12 nM; D_max: 91%). SMD-3040 TFA can inhibit tumor cell proliferation and exhibits antitumor activity. SMD-3040 TFA can be used in the study of tumors such as melanoma^[1]. (SMARCA2/4-ligand (HY-171765); HY-112078 VHL ligand (HY-112078))

In Vitro

SMD-3040 (0-1 μM; 1-48 h) TFA can effectively degrade SMARCA2 in cells such as Hela, SK-Mel-5 and SK-Mel-28^[1].
SMD-3040 (7 days) TFA significantly inhibits cell growth in SMARCA4-deficient cancer cell lines (such as SK-Mel-5, H838, A549, etc.) with GI₅₀ of 8.8-119 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	SK-Mel-5 and SK-Mel-28 cells
Concentration:	0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30, 100, 300 and 1000 nM
Incubation Time:	24 h
Result:	Inhibited the level of SMARCA2. Had DC₅₀ values of 20 and 35 nM in K-Mel-5 and SK-Mel-28 cells.

In Vivo

SMD-3040 (25-50 mg/kg; intravenous injection; twice weekly; two weeks) TFA exhibits antitumor activity in a xenograft mouse model of human melanoma^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Xenograft mouse models treated SK-Mel-5 melanoma cell line carrying SMARCA4 gene deletion^[1]
Dosage:	25 and 50 mg/kg
Administration:	Intravenous injection; twice weekly; two weeks
Result:	Effectively inhibited tumor growth in two different smarca4-deficient xenograft models. Was well-tolerated in mice.

Molecular Weight

1057.23

Formula

C₅₄H₆₇F₃N₁₀O₇S

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(N[C@H](C(N1[C@H](C(N[C@H](C2=CC=C(C3=C(C)N=CS3)C=C2)C)=O)C[C@@H](O)C1)=O)C(C)(C)C)[C@H]4CC[C@H](CN5CC6(CCC(N7N=CC(C8=C(N)N=NC(C9=C(O)C=CC=C9)=C8)=C7)CC6)C5)CC4.O=C(O)C(F)(F)F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (94.59 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9459 mL	4.7293 mL	9.4587 mL
5 mM	0.1892 mL	0.9459 mL	1.8917 mL
10 mM	0.0946 mL	0.4729 mL	0.9459 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (247 KB) HNMR (460 KB) RP-HPLC (134 KB) LCMS (132 KB)

Handling Instructions (2659 KB)

References

[1]. Yang L, et.al. Discovery of SMD-3040 as a Potent and Selective SMARCA2 PROTAC Degrader with Strong in vivo Antitumor Activity. J Med Chem. 2023 Jul 31. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.9459 mL	4.7293 mL	9.4587 mL	23.6467 mL
	5 mM	0.1892 mL	0.9459 mL	1.8917 mL	4.7293 mL
	10 mM	0.0946 mL	0.4729 mL	0.9459 mL	2.3647 mL
	15 mM	0.0631 mL	0.3153 mL	0.6306 mL	1.5764 mL
	20 mM	0.0473 mL	0.2365 mL	0.4729 mL	1.1823 mL
	25 mM	0.0378 mL	0.1892 mL	0.3783 mL	0.9459 mL
	30 mM	0.0315 mL	0.1576 mL	0.3153 mL	0.7882 mL
	40 mM	0.0236 mL	0.1182 mL	0.2365 mL	0.5912 mL
	50 mM	0.0189 mL	0.0946 mL	0.1892 mL	0.4729 mL
	60 mM	0.0158 mL	0.0788 mL	0.1576 mL	0.3941 mL
	80 mM	0.0118 mL	0.0591 mL	0.1182 mL	0.2956 mL

SMD-3040 TFA Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SMD-3040SMD3040SMD 3040PROTACsEpigenetic Reader DomainSMARCA2SMARCA4xenograft modelsPROTAC degraderSK-Mel-5H838A549Inhibitorinhibitorinhibit

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